Chemistry: analytical and immunological testing – Nitrogen containing – Amine and quaternary ammonium
Patent
1996-03-20
1998-06-09
Achutamurthy, Ponnathapura
Chemistry: analytical and immunological testing
Nitrogen containing
Amine and quaternary ammonium
435 4, 436149, 530395, G01N 3300, G01N 2700, C07K 120
Patent
active
057632766
ABSTRACT:
The invention provides a process for detecting the presence of guanidinium ions in a sample solution. The detection process involves contacting a sample solution suspected of containing guanidinium ions with a cation exchange resin and eluting the guanidinium ions present in the sample solution with an aqueous buffer solution having a pH of about 1.5 to about 2. This is followed by contacting the eluant with a cation suppressor column and simultaneously flowing a suppressor regenerate solution in the opposite direction on the opposite side of the permeable membrane of the column, and finally, detecting the presence of guanidinium ions in the eluant from the ion exchange column which was contacted with the suppressor column by use of a conductivity detector.
REFERENCES:
Krusius and Ruoslahti, "Primary structure of an extracellular matrix proteoglycan core protein deduced from cloned cDNA." Proc. Natl. Acad. Sci. USA, 83:7683-7687 (1986).
Ruoslahti, Erkki, "structure and biology of proteoglycene." Ann. Rev. Cell Biol., 4:229-255 (1988).
Choi et al., "Characterization of the dermatan sulfate proteoglycans, DS-PGI and DS-PGII, from bovine articular cartilage and skin isolated by octyl-sepharose chromatography." J. of Biol. Chem., 264(5):2876-2884 (1989).
Craig William S.
Harper John R.
Hernandez Sam D.
Kostel Paul J.
Parker Jonathan R.
Achutamurthy Ponnathapura
La Jolla Cancer Research Foundation
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