Processes for the production of HCMV glycoproteins, antibodies t

Details Processes for the production of HCMV glycoproteins, antibodies t Processes for the production of HCMV glycoproteins, antibodies t

1994-07-20

2000-12-19

Woodward, Michael P.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435400, 435476, 435 5, 4241471, 4242301, 536 2372, 514 44, 935 12, C12Q 170, G01N 3353, A61K 3929

Patent

active

061626201

ABSTRACT:
HCMV glycoproteins B and H have been identified. The gB protein is encoded by DNA in the HindIII F fragment of the HCMV genome lying between 1378 and 4095 bases from the F/D boundary. The gH protein is encoded by DNA in the HindIII L fragment lying between 228 and 2456 bases from the L/D boundary.
The genes have been incorporated in recombinant vaccinia vectors and expressed in host animals to raise HCMV-neutralising antibody, thereby indicating vaccine potential. The glycoproteins can also be used in a variety of different ways, as vaccines or in the production, purification or detection of HCMV antibody.

REFERENCES:
patent: 4689225 (1987-08-01), Pereira
patent: 4743562 (1988-05-01), Rasmussen et al.
patent: 4743848 (1988-05-01), Paoletti et al.
patent: 4762780 (1988-08-01), Spector et al.
patent: 5153311 (1992-10-01), Kari
patent: 5474914 (1995-12-01), Spaete et al.
patent: 5552143 (1996-09-01), Plotkin et al.
patent: 5834307 (1998-11-01), Spaete et al.
patent: 6100064 (2000-08-01), Burke et al.
Borysiewicz et al., "Human Cytomegalovirus-Specific Cytotoxic T Cells" J. Exp. Med. (1988) 168:919-931.
Britt, "Neutralizing Antibodies Detect a Disulfied-Linked Glycoprotein Complex Within the Envelope of Human Cytomegalovirus" Virology 135:369-378.
Cranage et al., "Identification and Expression of a Human Cytomegalovirus Glycoprotein with Homology to the Epstein-Barr Virus BXLF2 Product, Varicella-Zoster Virus gplll, and Herpes Simplex Virus Type I Glycoprotein H" J. of Virology (1988) 62:1416-1422.
Crange et al., Chemical Abstracts (1986) 106:No. 62013x.
Cranage et al., "Identification of the Human Cytomegalovirus Glycoprotein in B Gene and Induction of Neutralizing Antibodies Via its Expression in recombinant Vaccinia Virus" EMBO Journal (1986) 5:3057-3063.
Farrar et al., Vaccine (1986) 4:217-224.
Mach et al., "Mapping of the Major Glycoprotein Gene of Human Cytomegalovirus" J. Gen. Virol. (1986) 67:1461-1467.
Martiney et al. J. Virology (1986) 60:531-38.
Mocarski et al. "Precise localization of genes on large animal virus genomes: Use of .lambda.gt1 1 and monoclonal antibodies to map the gene for a cytomegalovirus protein family" PNAS USA (1985) 82:1266-1270.
Pande et al. "Cloning and physical mapping of a gene fragment coding for a 64-kilodalton major late antigen of human cytomegalovirus" Microbiology (1984) 81:4965-4969.
Pereira et al. "Monoclonal Antibodies to Human Cytomegalovirus: Three Surface Membrane Proteins With Unique Immunological and Electrophoretic Properties Specify Cross-Reactive Determinants" Infection and Immunity (1982) 36:924-932.
Pereira et al. "Cytomegalovirus-infected Cell Polypeptides Immune-Precipitated by Sera from Children with Congenital and Perinatal Infections" Infection and Immunity (1983) 39:100-108.
Pereira et al. "Polymorphism of Human Cytomegalovirus Glycoproteins Characterized by Monoclonal Antibodies" Virology (1984) 139:73-86.
Rasmussen et al. "Human Polypeptides Detected by a Complement-Dependent Neutralizing Murine Monoclonal Antibody to Human Cytomegalovirus" J. Virology (1985) 55:274-280.
Rasmussen et al. "Human Cytomegalovirus Polypeptides Stimulate Neutralizing Antibody In Vivo" Virology (1985) 145:186-190.
Suggs et al. "Use of synthetic oligonucleotides as hybridaiztion probes" PNAS (1981) 78:6613-6617.
European Search Report, Application No. EP 87 30 2001.
Curtsinger et al. J. Gen. Virol. 75:301-307, 1994.
Hopkins et al. J. Med. Virol. 49:124-131, 1996.
Berensci et al. Vaccine 14(5):369-374, 1996.
Harrison et al. V. Infect. Dis. 172:1212-1220, 1995.
Rapp et al. In Vivo Protection Studies with MCMV Glycoproteins . . . in Multidisciplinany Approach to Understanding Cytomegalovirus Disease (Elsevier Science Publishers) p. 327-332, 1995.
Watson et al Recombinant DNA: A Short Course "The Isolation of Cloned Genes", pp. 72-90, 1983.
Crainage et al, EMBO J. 5(11):3057-3063 (1986) "Id. of HCMV gB gene and Induction of Neutraliz.Ab . . . "

Affiliated with

Also associated with

Rating

Processes for the production of HCMV glycoproteins, antibodies t does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Processes for the production of HCMV glycoproteins, antibodies t, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Processes for the production of HCMV glycoproteins, antibodies t will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-270166