Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1987-03-02
1990-11-20
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041828, B01D 5702, G01N 2726
Patent
active
049716719
ABSTRACT:
A process for enabling the separation of a mixture of DNA fragments comprising (1) providing an electrophoresis devive; (2) adding to the device a solution mixture containing DNA fragments of different lengths; (3) energizing the device, thereby creating a sequence of unidirectional uniform electric field pulses therein, said sequence of field pulses alternating between primary positive voltage pulses and secondary pulses of a negative or a positive polarity with less voltage than the primary pulses; (4) calculating the time duration and the field strength required for the primary and secondary field pulses to enable resolution of the fragments into separate and distinct groups corresponding their lengths; and (5) applying in the device the selected primary and secondary fields with intensities and durations corresponding to the size of fragments to be separated. Another embodiment of the invention comprises a computer program that facilitates a DNA gel electrophoresis process.
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Noolandi Jaan
Slater Gary W.
Byorick Judith L.
Hsing Ben C.
Niebling John F.
Xerox Corporation
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