Processes for preparing conjugated linoleic acid from...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C554S126000, C554S223000, C554S224000

Reexamination Certificate

active

07138256

ABSTRACT:
Processes for preparing conjugated linoleic acid are described wherein a conjugated linoleic acid lower alkyl ester is subjected to hydrolysis in the presence of an enzyme to form a hydrolyzate comprising a conjugated linoleic acid and a lower alkanol, wherein at least a portion of the lower alkanol is continuously removed; the hydrolyzate is separated into an organic phase and an aqueous/alcoholic phase; and the conjugated linoleic acid is separated from the organic phase.

REFERENCES:
patent: 6184009 (2001-02-01), Cain et al.
patent: 6316645 (2001-11-01), Sih et al.
patent: 1 097 708 (2001-05-01), None
McNeill G P et al: “Enzymatic Enrichment of Conjugated Linoleic acid Isomers and Incorporation into Triglycerides” Journal of the American Oil Chemists' Society, American Oil Chemists' Society, Champaign, US, vol. 76, No. 11, Nov. 1999, pp. 1265-1628, XP001026473.
Haas M J et al: “Lipase-Catalyzed Fractionation of Conjugated Linoleic Acid Isomers” Lipids, Champaign, IL, US, vol. 34, No. 9, Sep. 1999, pp. 979-987, XP001056347 ISSN: 0024-4201.

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