Processes for making acylated insulin

Details Processes for making acylated insulin Processes for making acylated insulin

2008-07-22

2008-07-22

Gupta, Anish (Department: 1654)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

C514S002600, C530S300000, C530S303000

Reexamination Certificate

active

07402565

ABSTRACT:
A method is provided which allows high yields of acylated insulin. The method comprises expressing a singe-chain insulin precursor, preferably in yeast, cleaving the single-chain insulin precursor with a suitable protease which will open the peptide bond between the C-terminal amino group in the B-chain and a connecting peptide connecting the B chain with the A-chain, acylating the two-chain insulin intermediate in the ε-amino group in LysB29and subjecting the acylated two-chain insulin intermediate to a proteolytic enzyme which will cleave of the N-terminal extension on the B- and A-chains on the precursor molecule.

REFERENCES:
patent: 5646242 (1997-07-01), Baker et al.
patent: 5693609 (1997-12-01), Baker et al.
patent: 5750497 (1998-05-01), Havelund et al.
patent: 5905140 (1999-05-01), Hansen
patent: 5922675 (1999-07-01), Baker et al.
patent: 6011007 (2000-01-01), Havelund et al.
patent: RE37971 (2003-01-01), Baker et al.
patent: 163529 (1985-05-01), None
patent: 792290 (1994-09-01), None
patent: 1132404 (1994-09-01), None
patent: 712862 (1995-11-01), None
patent: 894095 (1997-02-01), None
patent: 96/29344 (1996-03-01), None
patent: 98/02460 (1997-07-01), None
patent: WO 02/079250 (2002-10-01), None
patent: 03/010186 (2003-02-01), None
Kjeldsen, T—Appl Microbiol Biotechnol—2000—vol. 54—pp. 277-286.
Kjeldsen, T et al—Prot Expres Purif—1998—vol. 14—pp. 309-316.
Kjeldsen, T et al—Gene—1996—vol. 170—pp. 107-110.

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