Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1983-08-11
1987-01-06
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 435240, 435948, 435811, 935 27, 935 31, 935 34, 935 56, 935 58, 935 70, C12P 2100, C12N 1500, C12N 500, C12R 191
Patent
active
046346651
ABSTRACT:
The present invention relates to processes for inserting DNA into eucaryotic cells, particularly DNA which includes a gene or genes coding for desired proteinaceous materials for which no selective criteria exist. The insertion of such DNA molecules is accomplished by cotransforming eucaryotic cells with such DNA together with a second DNA which corresponds to a gene coding for a selectable marker.
This invention also concerns processes for producing proteinaceous materials such as insulin, interferon protein, growth hormone and the like which involve cotransforming eucaryotic cells with DNA which codes for these proteinaceous materials, growing the contransformed cells for production of the proteinaceous material and recovering the proteinaceous material so produced.
The invention further relates to processes for inserting into eucaryotic cells a multiplicity of DNA molecules which includes genes coding for desired proteinaceous materials. The insertion of multiple copies of desired genes is accomplished by cotransformation with the desired genes and with amplifiable genes for a dominant selectable marker in the presence of successively higher amounts of an inhibitor. Alternatively, the insertion of multiple copies of desired genes is accomplished by transformation using DNA molecules formed by ligating a DNA molecule including the desired gene to a DNA molecule which includes an amplifiable gene coding for a dominant selectable phenotype such as a gene associated with resistance to a drug in the presence of successively higher amounts of an agent such as a drug against which the gene confers resistance so that only those eucaryotic cells into which multiple copies of the amplifiable gene have been inserted survive. Eucaryotic cells into which multiple copies of the amplifiable gene have been inserted additionally include multiple copies of the desired gene and may be used to produce multiple copies of proteinaceous molecules. In this way otherwise rare proteinaceous materials may be obtained in higher concentrations than are obtainable using conventional techniques.
REFERENCES:
Numberg et al, Proc. Nat'l. Acad. Sci., U.S.A., 75(11):5553-5556 (1978).
Wigler et al, Cell, 11:223-232 (1977).
Kretschmer et al, J. Bacteriol., 124(1):225-231 (1975).
Wigler et al, Cell, 14:725-731, (1978).
Wigler et al, Cell, 16:777-785 (Apr. 1979).
Wold et al, Proc. Nat'l. Acad. Sci., U.S.A., 76(11):5684-5688 (1979).
Pellicer et al, Proc. Nat'l. Acad. Sci., U.S.A., 77(4):2098-2102 (Apr. 1980).
Willecke et al, Proc. Nat'l. Acad. Sci., U.S.A., 73(4):1274-1278 (1976).
Lai et al, Proc. Nat'l. Acad. Sci., U.S.A., 77(1):244-248 (Jan. 1980).
Wigler et al, Eucaryotic Gene Regulation Proc. Inc.-UCLA Symposia, R. Axel and T. Maniatis, Editors, Academic Press, 457-475 (1979).
Miller et al, Proc. Nat'l. Acad. Sci., U.S.A., 75(7):3346-3350 (1978).
Axel Richard
Silverstein Saul J.
Wigler Michael H.
Kepplinger Esther M.
The Trustees of Columbia University in the City of New York
White John P.
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