Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Reexamination Certificate
2009-01-09
2011-11-15
Jiang, Shaojia Anna (Department: 1623)
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
C536S025300
Reexamination Certificate
active
08058448
ABSTRACT:
The present invention relates to processes and reagents for oligonucleotide synthesis and purification. One aspect of the present invention relates to compounds useful for activating phosphoramidites in oligonucleotide synthesis. Another aspect of the present invention relates to a method of preparing oligonucleotides via the phosphoramidite method using an activator of the invention. Another aspect of the present invention relates to sulfur-transfer agents. In a preferred embodiment, the sulfur-transfer agent is a 3-amino-1,2,4-dithiazolidine-5-one. Another aspect of the present invention relates to a method of preparing a phosphorothioate by treating a phosphite with a sulfur-transfer reagent of the invention. In a preferred embodiment, the sulfur-transfer agent is a 3-amino-1,2,4-dithiazolidine-5-one. Another aspect of the present invention relates to compounds that scavenge acrylonitrile produced during the deprotection of phosphate groups bearing ethylnitrile protecting groups. In a preferred embodiment, the acrylonitrile scavenger is a polymer-bound thiol. Another aspect of the present invention relates to agents used to oxidize a phosphite to a phosphate. In a preferred embodiment, the oxidizing agent is sodium chlorite, chloramine, or pyridine-N-oxide. Another aspect of the present invention relates to methods of purifying an oligonucleotide by annealing a first single-stranded oligonucleotide and second single-stranded oligonucleotide to form a double-stranded oligonucleotide; and subjecting the double-stranded oligonucleotide to chromatographic purification. In a preferred embodiment, the chromatographic purification is high-performance liquid chromatography.
REFERENCES:
patent: 3276950 (1966-10-01), Engelhard et al.
patent: 3520897 (1970-07-01), Glentworth et al.
patent: 3621030 (1971-11-01), Seltzer
patent: 3732239 (1973-05-01), Spatz et al.
patent: 3740396 (1973-06-01), Haugwitz et al.
patent: 3753908 (1973-08-01), de Vries et al.
patent: 3956303 (1976-05-01), Bullock et al.
patent: 4240822 (1980-12-01), Diehl et al.
patent: 4474948 (1984-10-01), Hudson et al.
patent: 5677124 (1997-10-01), DuBois et al.
patent: 5750672 (1998-05-01), Kempe
patent: 5919625 (1999-07-01), DuBois et al.
patent: 5939262 (1999-08-01), Pasloske et al.
patent: 6057134 (2000-05-01), Lader et al.
patent: 6096881 (2000-08-01), Han et al.
patent: 6232103 (2001-05-01), Short
patent: 6399334 (2002-06-01), Li et al.
patent: 6593464 (2003-07-01), Gebeyehu et al.
patent: 6610490 (2003-08-01), Schuster et al.
patent: 6620926 (2003-09-01), Sproat
patent: 6623962 (2003-09-01), Akhtar et al.
patent: 6649751 (2003-11-01), Usman et al.
patent: 6673611 (2004-01-01), Thompson et al.
patent: 6673918 (2004-01-01), Bellon et al.
patent: 6686463 (2004-02-01), Beigelman et al.
patent: 6797815 (2004-09-01), Matulic-Adamic et al.
patent: 6815205 (2004-11-01), Lin et al.
patent: 6818447 (2004-11-01), Pavco et al.
patent: 6818759 (2004-11-01), Beigelman et al.
patent: 6830902 (2004-12-01), Astatke et al.
patent: 6849726 (2005-02-01), Usman et al.
patent: 6933121 (2005-08-01), Schuster et al.
patent: 6972330 (2005-12-01), Beigelman et al.
patent: 6977295 (2005-12-01), Belotserkovskii et al.
patent: 2002/0025526 (2002-02-01), Schuster et al.
patent: 2002/0034750 (2002-03-01), Short
patent: 2002/0086356 (2002-07-01), Tuschl et al.
patent: 2002/0120129 (2002-08-01), Beigelman et al.
patent: 2002/0132346 (2002-09-01), Cibelli
patent: 2002/0143166 (2002-10-01), Pires et al.
patent: 2002/0162126 (2002-10-01), Beach et al.
patent: 2002/0182590 (2002-12-01), Strange et al.
patent: 2003/0084471 (2003-05-01), Beach et al.
patent: 2003/0108923 (2003-06-01), Tuschl et al.
patent: 2003/0143732 (2003-07-01), Fosnaugh et al.
patent: 2003/0148507 (2003-08-01), Fosnaugh et al.
patent: 2003/0157030 (2003-08-01), Davis et al.
patent: 2003/0167490 (2003-09-01), Hunter et al.
patent: 2003/0170891 (2003-09-01), McSwiggen
patent: 2003/0175950 (2003-09-01), McSwiggen
patent: 2003/0190635 (2003-10-01), McSwiggen
patent: 2003/0190661 (2003-10-01), Gruber et al.
patent: 2003/0204077 (2003-10-01), Simms
patent: 2003/0206887 (2003-11-01), Morrissey et al.
patent: 2004/0009522 (2004-01-01), Wu
patent: 2004/0009946 (2004-01-01), Lewis et al.
patent: 2004/0014113 (2004-01-01), Yang et al.
patent: 2004/0018181 (2004-01-01), Kufe et al.
patent: 2004/0018999 (2004-01-01), Beach et al.
patent: 2004/0019001 (2004-01-01), McSwiggen
patent: 2004/0044190 (2004-03-01), Sproat
patent: 2004/0058886 (2004-03-01), Scaringe
patent: 2004/0063654 (2004-04-01), Davis et al.
patent: 2004/0077574 (2004-04-01), Klinghoffer et al.
patent: 2004/0086884 (2004-05-01), Beach et al.
patent: 2004/0121353 (2004-06-01), Lewis et al.
patent: 2004/0138163 (2004-07-01), McSwiggen et al.
patent: 2004/0142895 (2004-07-01), Lockridge et al.
patent: 2004/0147735 (2004-07-01), Laurent et al.
patent: 2004/0161777 (2004-08-01), Baker et al.
patent: 2004/0191905 (2004-09-01), Stevenson et al.
patent: 2004/0192626 (2004-09-01), McSwiggen et al.
patent: 2004/0192629 (2004-09-01), Xu et al.
patent: 2004/0198640 (2004-10-01), Leake et al.
patent: 2004/0198682 (2004-10-01), McSwiggen et al.
patent: 2004/0203024 (2004-10-01), Baker et al.
patent: 2004/0203145 (2004-10-01), Zamore et al.
patent: 2004/0204420 (2004-10-01), Rana
patent: 2004/0209831 (2004-10-01), McSwiggen et al.
patent: 2004/0209832 (2004-10-01), McSwiggen et al.
patent: 2004/0214198 (2004-10-01), Rana
patent: 2004/0219671 (2004-11-01), McSwiggen et al.
patent: 2004/0220128 (2004-11-01), Paveco et al.
patent: 2004/0224405 (2004-11-01), Leake et al.
patent: 2004/0229266 (2004-11-01), Tuschl et al.
patent: 2004/0234504 (2004-11-01), Verma et al.
patent: 2004/0235775 (2004-11-01), Kung et al.
patent: 2004/0249178 (2004-12-01), Vargeese et al.
patent: 2004/0259247 (2004-12-01), Tuschl et al.
patent: 2004/0259248 (2004-12-01), Tuschl et al.
patent: 2004/0266707 (2004-12-01), Leake et al.
patent: 2005/0004063 (2005-01-01), Kung et al.
patent: 2005/0014172 (2005-01-01), Richards et al.
patent: 2005/0020525 (2005-01-01), McSwiggen et al.
patent: 2005/0026278 (2005-02-01), Tuschl et al.
patent: 2005/0032733 (2005-02-01), McSwiggen et al.
patent: 2005/0042646 (2005-02-01), Davidson et al.
patent: 2005/0048529 (2005-03-01), McSwiggen
patent: 2005/0054596 (2005-03-01), McSwiggen et al.
patent: 2005/0054598 (2005-03-01), McSwiggen
patent: 2005/0054847 (2005-03-01), Madden et al.
patent: 2005/0059817 (2005-03-01), Beigelman et al.
patent: 2005/0070497 (2005-03-01), McSwiggen et al.
patent: 2005/0075304 (2005-04-01), McSwiggen et al.
patent: 2005/0079610 (2005-04-01), Polisky et al.
patent: 2005/0096284 (2005-05-01), McSwiggen
patent: 2005/0106726 (2005-05-01), McSwiggen et al.
patent: 2005/0119211 (2005-06-01), Chowrira et al.
patent: 2005/0119212 (2005-06-01), Haeberli et al.
patent: 2005/0124566 (2005-06-01), Robin et al.
patent: 2005/0124567 (2005-06-01), McSwiggen et al.
patent: 2005/0124568 (2005-06-01), Usman et al.
patent: 2005/0124569 (2005-06-01), Guerciolini et al.
patent: 2005/0130181 (2005-06-01), McSwiggen
patent: 2005/0136436 (2005-06-01), McSwiggen et al.
patent: 2005/0137153 (2005-06-01), McSwiggen et al.
patent: 2005/0137155 (2005-06-01), McSwiggen et al.
patent: 2005/0142578 (2005-06-01), Usman et al.
patent: 2005/0143333 (2005-06-01), Richards et al.
patent: 2005/0148530 (2005-07-01), McSwiggen et al.
patent: 2005/0153914 (2005-07-01), McSwiggen et al.
patent: 2005/0153915 (2005-07-01), Usman et al.
patent: 2005/0153916 (2005-07-01), McSwiggen et al.
patent: 2005/0158735 (2005-07-01), McSwiggen et al.
patent: 2005/0159376 (2005-07-01), McSwiggen et al.
patent: 2005/0159378 (2005-07-01), McSwiggen et al.
patent: 2005/0159379 (2005-07-01), McSwiggen et al.
patent: 2005/0159380 (2005-07-01), Guerciolini et al.
patent: 2005/0159381 (2005-07-01), McSwiggen et al.
patent: 2005/0159382 (2005-07-01), McSwiggen et al.
patent: 2005/0164224 (2005-07-01), McSwiggen et al.
patent: 2005/0164966 (2005-07-01), McSwiggen et al.
patent: 2005/0164967 (2005-07-01), McSwiggen et al.
patent: 2005/0164968 (2005-07-01), McSwiggen et al.
patent:
Jung Michael E.
Manoharan Muthiah
Pandey Rajendra K.
Rajeev Kallanthottathil G.
Wang Gang
Alnylam Pharmaceuticals, Inc.
Jiang Shaojia Anna
Lau Jonathan S
LeClairRyan
Townes Jeffrey N.
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