Processes and devices for detection of substances such as enzyme

Details Processes and devices for detection of substances such as enzyme Processes and devices for detection of substances such as enzyme

1981-08-13

1983-10-25

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 15, 435 23, 435174, 435199, 435197, 435213, 435291, 435807, 435 4, C12N 912, C12N 918, C12N 976, C12N 1100, C12Q 146, C12Q 148, C12Q 138, C12M 134

Patent

active

044119895

ABSTRACT:
The disclosed methods and devices for detecting or monitoring or identifying substances (such as chemical warfare agents) and residual environmental pollutants (such as pesticides) utilize the discovery that spectra (e.g. infrared absorption spectra) of an uninhibited enzyme (e.g., a cholinesterase) can differ from spectra of the same enzyme which has been complexed with the agent pollutant. For example, the infrared spectrum of uninhibited butyrylcholinesterase (BuChE) lacks a distinct absorption peak found at about 1000 cm.sup.-1 in the BuChE-Malathion spectrum. The enzyme is used to collect and concentrate the agent or pollutant, and the resulting complexed enzyme can then be analyzed (e.g., by infrared spectroscopy) and its spectrum compared to an uninhibited enzyme spectrum.
Relatively simple devices can carry out the collection and detection or monitoring or identification steps of this invention, given appropriate models of complexed and uncomplexed enzyme specta upon which to base the design of the devices.

REFERENCES:
patent: 3049411 (1962-08-01), Gelman et al.
patent: 3081158 (1963-03-01), Winter
patent: 3378463 (1968-04-01), Guilbault et al.
patent: 3451901 (1969-06-01), Seiger et al.
patent: 3515644 (1970-06-01), Kramer et al.
patent: 3526480 (1970-09-01), Findl et al.
patent: 3539452 (1970-11-01), Penicnak
patent: 3689224 (1972-09-01), Agnew et al.
patent: 3715298 (1973-02-01), Goodson et al.
patent: 3730841 (1973-05-01), Salvatore et al.
patent: 3741876 (1973-06-01), Guilbault et al.
patent: 3809616 (1974-05-01), Schmitt et al.
patent: 3912704 (1975-10-01), Singh
patent: 4059491 (1977-11-01), Iwasa et al.
patent: 4324858 (1982-04-01), Goodson et al.
Makinen et al., Ann. Rev. Biophys. Bioeng. 6:301 (1977), pp. 301-343.

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