Process to obtain monoclonal and polyclonal antibodies to identi

Details Process to obtain monoclonal and polyclonal antibodies to identi Process to obtain monoclonal and polyclonal antibodies to identi

1990-08-15

1997-08-26

Feisee, Lila

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

4242651, 530412, C12P 2108, C07K 114, A61K 39002

Patent

active

056610106

ABSTRACT:
The present invention is a process to obtain polyclonal and monoclonal antibodies useful to identify symptomatic or asymptomatic amebiasis. This invention includes the following steps: 1) Antigen preparation from electron dense granules (EDGs) secreted by Entamoeba histolytica trophozoites incubated in medium containing collagen type I; 2) EDG purification by differential centrifugation and 3) Production of polyclonal and monoclonal antibodies. Anti-EDG polyclonal antibodies were prepared from the serum of a goat hyperimmunized with EDGs. Monoclonal antibodies were obtained by fusion of spleen cells from Balb/c mice injected with EDG protein and SP2/O-Ag14 cells.

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