Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1990-10-19
1993-08-24
Chereskin, Che S.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352404, 43524049, 47DIG9, 47 58, A01H 400, A01H 510
Patent
active
052388352
ABSTRACT:
A process for inducing desiccation tolerance in an in vitro formed plant embryo to enable production of viable artificial seeds is disclosed. The process comprises culturing in vitro plant embryos, stimulating the embryos to proceed through globular and a heart shaped stage into an elongate-torpedo shaped stage and early cotyledon stage, inducing the embryos as early as the torpedo-shaped stage with a source of abscisic acid at an effective concentration of abscisic acid and for a sufficient period of time to cause expression of desiccation tolerance which includes change in cellular metabolism, electron transport processes and oxidation-reduction reactions in the embryos and drying the induced embryos to provide stable viable embryos.
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Bewley J. Derek
Bowley Steve
Brown Daniel C. W.
McKersie Bryan D.
Senaratna Tissa
Chereskin Che S.
University of Guelph
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