Process to determine enzyme activity

Details Process to determine enzyme activity Process to determine enzyme activity

2011-02-22

2011-02-22

Carlson, Karen Cochrane (Department: 1657)

Electric lamp and discharge devices

Having vapor generating material

Reexamination Certificate

active

07893616

ABSTRACT:
The present invention provides for various processes for determining enzyme activity. In one embodiment, a process for detecting an enzyme activity includes contacting a blood component with a substrate comprising a cleavage site of the enzyme and a tag moiety to produce a sample comprising a cleavage product having a known mass, and placing the sample in contact with a SELDI sample chip which has surface moieties which bind to the cleavage product. In another embodiment, the process further includes subjecting the SELDI sample chip to SELDI mass spectrometry to identify the cleavage product. The present invention also provides for a kit which includes a SELDI sample chip having surface moieties capable of binding to an enzyme cleavage product.

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ADAMTS 13 Activity Assay 1 pg., 2007.
Wu, Haifeng, et al., Application of SELDI-TOF Mass Spectrometry in Clinical Evaluation of Thrombotic Thrombocytopenic Purpura. pp. 1-19.
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Cataland, S.R., Full Evaluation of an Acquired Case of Thrombotic Thrombocytopenic Purpura Following the Surgical Resection of Glioblastoma Multiforme. pp. 2733-2737.
ADAMTS13 Activity Assay (Low Curve). Feb. 15, 2007, pp. 1-2.
Jin, M., et al. A Rapid Test for the Diagnosis of Thrombotic Thrombocytopenic Purpura Using Surface Enhanced Laser Desorption/Ionization Time-of-Fligh (SELDI-TOF)-Mass Spectrometry. Journal of Thrombosis and Haemostasis, 4: 333-338, 2005, International Society on Thrombosis and Haemostasis.
Cataland, S.R., An Evaluation of Cyclosporine Alone for the Treatment of Early Recurrences of Thombotic Thrombocytopenic Purpura. pp. 1162-1164, 2006, International Society on Thrombosis and Haemostasis.

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