Process of using light absorption to control enzymatic depolymer

Details Process of using light absorption to control enzymatic depolymer Process of using light absorption to control enzymatic depolymer

1987-04-29

1992-04-21

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435174, 435177, 435803, 435813, 514 56, 536 21, C12P 1926, C12N 1100, A61K 31725, C08B 3710

Patent

active

051067340

ABSTRACT:
A method is provided for controlling production of low molecular weight heparin (LMW-heparin) when depolymerizing heparin with heparinase in a reaction mixture in a reactor. Depolymerization to a desired average molecular weight is monitored by measuring an increase in UV-absorption (preferably at 230-235). When the absorption has reached a value for a desired molecular weight, the depolymerization is stopped or LMW-heparin having the desired molecular weight is continuously removed from the reaction mixture. In a preferred embodiment, the reaction mixture is subjected to ultrafiltration to produce a filtrate containing LMW-heparin, and a retentate which is recycled to the reactor. UV-absorption and refractive index of the filtrate are measured, and depolymerization is controlled in accordance with the measured absorption and refractive index to produce a filtrate containing a LMW-heparin of low polydispersity and predetermined molecular weight.

REFERENCES:
patent: 4396762 (1983-08-01), Langer et al.
Deeslie, et al., J. Food. Sci., vol. 46, No. 4, 1981, pp. 1035-1042.
Porter, et al., Chem. Tech., Jan. 1971, pp. 56-63.

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