Process of using bacteria that metabolize phenylacetate through

Details Process of using bacteria that metabolize phenylacetate through Process of using bacteria that metabolize phenylacetate through

1991-03-11

1992-09-29

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435 42, 435142, 435146, 435170, 435232, 4352521, 43525234, 4352533, 435877, C12P 3900, C12P 724, C12N 988, C12R 140

Patent

active

051513536

ABSTRACT:
Bacteria that metabolize phenylacetate along a mandelate-to-cis,cis-muconate pathway are disclosed. Bacteria that express the pathway for metabolizing phenylacetate through a mandelate intermediate can be isolated reproducibly by first selecting bacteria that can utilize both L-phenylalanine and mandelate as the sole carbon source and then blocking alternate pathways for the degradation of L-phenylalanine. The activity of selected enzymes along the mandelate-to-cis,cis-muconate pathway can be blocked in these bacteria to effect the accumulation of selected intermediates.

REFERENCES:
Kennedy et al, Biochem. J. (1968) vol. 107, pp. 497-506.
"Enzyme Nomenclature" 1984 Academic Press 1984, pp. 394-395.

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