Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1987-09-17
1991-01-15
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435212, 435219, 435815, C12N 964, C12N 950
Patent
active
049853627
ABSTRACT:
Tissue plasminogen activator (tPA) species having a molecular weight of about 70,000 daltons is isolated in a purified form from a crude tPA preparation containing various tPA species having different molecular weights by bringing the crude tPA preparation into contact with a cation exchanger in advance and separating the desired tPA species selectively from the cation exchanger by means of the salt gradient elution method.
REFERENCES:
patent: 4552760 (1985-11-01), Murakami et al.
patent: 4568544 (1986-02-01), Hasegawa et al.
Rijken, D. et al., J. Biol. Chem., vol. 256, pp. 7035-7041, 1981.
Pharmacia-Ion Exchange Chromatography, Principles & Methods, pp. 18-22, 1980.
"Human Tissue-Type Plasmsnogen Activator", Kruithof et al; Biochem. J., (1985).
Pharmacia-Ion Exchange Chromatography, Principles & Methods, pp. 29-38, 1983.
Kawashima Nobuhiro
Mori Kunizou
Morii Mitsuyoshi
Morii Noriko
Ohoka Masaharu
Mitsui Toatsu Chemicals Incorporated
Naff David M.
Patterson Jr. Charles L.
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