Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1994-12-29
1997-01-14
Walsh, Stephen G.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530350, 435 697, 4351723, 436525, 536 231, 536 234, 536 235, G01N 33553, C12P 2104
Patent
active
055941150
ABSTRACT:
Recombinant fusion proteins which comprise a biologically active polypeptide portion and metal chelating peptide are disclosed. The fusion proteins contain metal chelating peptides which have at least three and preferably six alternating histidine residues. Fusion proteins which contain metal chelating peptides that are substrates for dipeptidylpeptidase I are also disclosed. Additionally, a method of obtaining desired polypeptides by producing recombinant fusion proteins and purifying them with immobilized metal ions is disclosed. A kit for purifying desired polypeptides is also disclosed.
REFERENCES:
patent: 4569794 (1986-02-01), Smith
patent: 4751180 (1988-06-01), Cousens
patent: 4782137 (1988-11-01), Hopp
patent: 5202259 (1993-04-01), Goff et al.
Sharma et al. 1992. Methods: A Companion to Methods in Enzymology 4:57-67.
Hochuli et al. 1988 Bio/Technology 6:1321-1325.
Smith, M. C. et al., "Chelating Peptide-Immobilzed Metal Ion Affinity Chromatography", J. Biol. Chem. 263(15):7211-7215, 1988.
Hochuli, E., et al., "New Metal Chelate Adsorbent Selective for Proteins and Peptides Containing Neighbouring Histidine Residues", J. Chromat. 411:177-184, 1987.
Ljungquist, C., et al. "Immobilization and Affinity Purification of Recombinant Proteins Using Histidine Peptide Fusions", Eur. J. Biochem. 186:563-569, 1989.
Haffey, M. L. et al., "Site-Specific Cleavage of a Fusion Protein by Renin", DNA, 6(6):565-571, 1987.
Hopp, T. P. et al., "A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification", Bio/Technol. 6:1204-1210, Oct. 1988.
Kreil, G., "Processing of Precursors by Dipeptidylaminopeptidases: A Case of Molecular Ticketing", TIBS 15:23-26, Jan. 1990.
Dalb.o slashed.ge, H. et al., "A Novel Enzymatic Method for Production of Authentic hGH from an Escherichia Coli Produced hGH-Precursor", Bio/Technol 5:161-164, Feb. 1987.
Dalb.o slashed.ge, H. et al., "Cloning and Expression of an Interleukin-1.beta. Precursor and Its Conversion to Interleukin-1.beta.", FEBS 246(1,2):89-93, Mar. 1989.
Dalb.o slashed.ge, H., et al., "In vivo Processing of N-Terminal Methionine in E. Coli", FEBS 266(1,2):1-3, Jun. 1990.
McDonald, J. K., et al., J. Biol. Chem. 244(10):2693-2709, May 1969.
Darnley, Jr. James D.
DeLuca Mark
Kemmerer Elizabeth E.
Pharmacia & Upjohn Company
Walsh Stephen G.
LandOfFree
Process of purifying recombinant proteins and compounds useful i does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process of purifying recombinant proteins and compounds useful i, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process of purifying recombinant proteins and compounds useful i will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1389248