Process of producing modified superoxide dismutase

Details Process of producing modified superoxide dismutase Process of producing modified superoxide dismutase

1993-12-03

1995-04-04

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435180, 435189, 435190, C12N 1108, C12N 1106, C12N 902, C12N 904

Patent

active

054037310

ABSTRACT:
A modified superoxide dismutase represented by the formula: ##STR1## (wherein R is as defined below; SOD is a residue of superoxide dismutase) is produced, in a shortened period of reaction with a high and constant modification ratio, by reacting a polymeric carbonyldiimidazole derivative represented by the formula: ##STR2## (wherein R is a residue of a water soluble polymer having an average molecular weight of about 2,000 to 10,000) with superoxide dismutase in the presence of a buffer having a pH of 9.0 to 11.0 and a concentration of 0.1 M to 0.5 M, preferably 0.2 M to 0.4 M, at a temperature of 30.degree. to 70.degree. C., preferably 45.degree. to 60.degree. C. The modified superoxide dismutase thus produced exhibits a suitably prolonged blood half-life.

REFERENCES:
patent: 4496689 (1985-01-01), Mitra
patent: 4563349 (1986-01-01), Miyata et al.
patent: 4774185 (1988-09-01), Asami et al.
patent: 5006333 (1991-04-01), Saifer et al.
Beauchamp et al. (1983) Anal. Biochem., 131, 25-33.
Sanchez-Moreno (1989) Arch. Microbiol., 152, 407-410, "Superoxide Dismutase in Strains of the genus Flavobacterium: Isolation and Characterization".

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