Process of producing highly transformable bacterial cells and ce

Details Process of producing highly transformable bacterial cells and ce Process of producing highly transformable bacterial cells and ce

1996-04-18

1998-01-13

Degen, Nancy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

43525233, 4352528, C12N 1564, C12N 120, C12N 121

Patent

active

057078416

ABSTRACT:
The invention provided herein includes gram negative bacteria cells containing a gene encoding an enzyme with carbohydrate degrading activity that had been rendered competent to transformation. Carbohydrate degrading enzymes of interest for use in the invention include alpha-amylase. The competent cells of the subject invention may be frozen so as to provide for prolonged storage. Other aspects of the invention include methods for rendering gram negative bacterial cells, such as E. coli cells competent to transformation. These methods involve the step of transferring a gene encoding an enzyme with carbohydrate degrading activity into E. coli cells and subsequently rendering the cells competent using any of a variety of competency inducing procedures.

REFERENCES:
patent: 4493893 (1985-01-01), Mielenz et al.
patent: 5512468 (1996-04-01), Greener
Hanahan, D. 1983. J. Mol. Biol. vol. 166, pp. 557-580 "Studies on Transformation of E. coli with Plasmids".
Studier, F.W. 1991. J. Mol. Biol. vol. 219 pp.37-44. "Use of Bacteriophage T7 Lysozyme to Improve an Inducible T7 . . . ".
Cornelis et al., 1982, "Cloning and expression of a Bacillus coagulans amylase gene in Escherichia coli," Mol. Gen. Genet. 186(4):507-511.
Tsukagoshi et al., 1984, "Cloning and Expression of a Thermophilic .alpha.-Amylase Gene from Bacillus stearothermophilus in Escherichia coli," Mol. Gen. Genet. 193:58-63.
Antranikian et al., 1987, "Changes in the cell envelope structure of Clostridium sp. strain EM1 during massive production of alpha-amylase and pullulanase," FEMS Microbiology Letters 41:193-197.
Oriel and Schwacha, 1988, "Growth on starch and extracellular production of thermostable amylase by Escherichia coli," Enzyme Microb. Technol. 10:42-46.

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