Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-04-26
1996-02-06
Benzion, Gary
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351721, 43524045, 4352405, 800205, 800DIG56, 536 237, C12N 1505, A01H 400
Patent
active
054895200
ABSTRACT:
This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
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Adams Thomas R.
Chambers Sheryl A.
Daines Richard J.
Gordon-Kamm William J.
Kausch Albert P.
Benzion Gary
Dekalb Genetics Corporation
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