Process of making cyclodextrin glucanotransferase with brevibact

Details Process of making cyclodextrin glucanotransferase with brevibact Process of making cyclodextrin glucanotransferase with brevibact

1995-06-06

1998-01-13

Marx, Irene

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435200, 435169, 435840, C12P 2104

Patent

active

057078335

ABSTRACT:
This invention relates to a .gamma.-cyclodextrin glucanotransferase having novel properties, to a process for the production of the .gamma.-cyclodextrin glucanotransferase which comprises culturing a strain belonging to the genus Brevibacterium capable of producing cyclodextrin glucanotransferase, thereby allowing the strain to produce the .gamma.-cyclodextrin glucanotransferase in a culture medium, and subsequently collecting the enzyme, to a process for the production of cyclodextrin which comprises allowing the cyclodextrin glucanotransferase to react with substrate dissolved in a solution, thereby effecting formation of .gamma.-cyclodextrin as a main product, and to a method for increasing .gamma.-cyclodextrin yield without accompanying yield increase of total cyclodextrin, which comprises adding ethyl alcohol to a reaction solution in which .gamma.-cyclodextrin and .beta.-cyclodextrin are formed from starch by the action of .gamma.-cyclodextrin glucanotransferase, thereby effecting repressed formation of .beta.-cyclodextrin and enhanced formation of .gamma.-cyclodextrin. Development of the process of this invention has rendered possible low cost production of .gamma.-cyclodextrin and therefore expands practical use of .gamma.-cyclodextrin to the field of food and the like.

REFERENCES:
Bender, H, Arch Microbiol, vol. 110, pp. 271-282, 1977.
Goodfellow et al., "Biology of the Actinomycetes", 1984, Academic Press, pp. 44-46, 77-79.
Database WPI, Week 8107, Derwent Publications Ltd., AN 81-10404D, Dec. 1980.
Database WPI, Week 8710, Derwent Publications Ltd., AN 87-070684, Feb. 1987.
Database WPI, Week 8703, Derwent Publications Ltd., AN 87-017596, Dec. 1986.
Fujita et al., Purification and Properties of Cyclodextrin Glycosyltransferase from Bacillus sp. AL-6, Journal of Fermentation and Bioengineering, vol. 70, No. 3, pp. 150-154, 1990.
Tomita et al., Some Factors Affecting the Formation of .gamma.-Cyclodextrin Using Cyclodextrin -Glycosyltransferase from Bacillus sp. AL-6, Journal of Fermentation and Bioengineering, vol. 70, No. 3, pp. 190-192, 1990.
Kato and Horikoshi, A New .gamma.-Cyclodextrin Forming Enzyme Produced by Bacillus subtilis No. 313, J. Jpn. Soc. Starch Sci., 33, pp. 137-143, 1986. Abstract only.
Schmid et al., New Trends in Cyclodextrins and Derivatives, pp. 25-54, 1991 .

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