Process of increasing cellular production of biologically...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S183000, C435S193000, C435S252300

Reexamination Certificate

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07638306

ABSTRACT:
A process of increasing the cellular production of biologically active compounds is provided. The process is particularly useful for increasing antibiotic production by bacterial cells. The process includes the step of inhibiting the activity of methylmalonyl-CoA mutase.

REFERENCES:
Vrijbloed et al. Insertional inactivation of methylmalonyl coenzyme A (CoA) mutase and isobutyryl-CoA mutase genes inStreptomyces cinnamonensis: influence on polyketide antibiotic biosynthesis, J Bacteriol. Sep. 1999; 181(18): 5600-5.
Katz et al. Novel macrolides through genetic engineering, Med Res Rev. Nov. 1999; 19(6): 543-58. Review.
Whisstock et al. Quaterly Reviews of Biophysics, 2003, “Prediction of protein function from protein sequence and structure”, 36(3): 307-340.
Branden et al. Introduction to protein structure, Gerald Publishing Inc., New York, p. 247, 1991.
Witkowski et al. Conversion of a beta-ketoacyl synthase to a malonyl decarboxylase by replacement of the active-site cysteine with glutamine, Biochemistry. Sep. 7, 1999;38(36):11643-50.
Seffernick et al. Melamine deaminase and atrazine chlorohydrolase: 98 percent identical but functionally different, J Bacteriol. Apr. 2001;183(8):2405-10.
T. E. Weber, A. P. Schinckel, K. L. Houseknecht and B. T. Richert. 2001. Evaluation of conjugated linoleic acid and dietary antibiotics as growth promotants in weanling pigs. J Anim Sci. 79:2542-2549.
Aparicio, JF, Cafrey P, Gil JA, Zotchev SB. Polyene antibiotic biosynthesis gene clusters. Appl Microbiol Biotechnol. May 2003;61(3):179-88. Epub 2002 Dec 18.
Aparicio JF, Molnar I, Schwecke T, Konig A, Haydock SF, Khaw LE, Staunton J, Leadleyγ PF. Organization of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of the enzymatic domains in the modular polyketide synthase. Gene. Feb 22, 1996;169(1):9-16.
Bibb MJ, White J, Ward JM, Janssen GR. The mRNA for the 23S rRNA methylase encoded by the ermE gene of Saccharopolyspora erythraea is translated in the absence of a conventional ribosome-binding site. Mol Microbiol. Nov. 1994;14(3):533-45.
Birch a, Leiser A, Robinson JA. Cloning, sequencing, and expression of the gene encoding methylmalonylcoenzyme A 20 mutase from Streptomyces cinnamonensis. J Bacteriol. Jun. 1993;175(11):3511-9.
Dayem LC, Carney JR, SantI DV, Pfeifer BA, Khosla C, Kealey JT. Metabolic engineering of a methylmalonyl-CoA mutase-epimerase pathway for complex polyketide biosynthesis in Escherichia coli. Biochemistry. Apr. 2002 23;41(16):5193-201.
Donadio S, Stayer MJ, Katz L. Erythromycin production in Saccharopolyspora erythraea does not require a functional propionyl-CoA carboxylase. Mol Microbiol. mar. 1996;19(5):977-84.
Dotzlaf JE, Metzger LS, Fogelsong MA. Incorporation of amino acid-derived carbon into tylactone by Streptomyces fradiae GS 14. Antimicrob Agents Chemother. Feb. 1984;25(2):216-20.
Fleischmann RD, Adams MD, White 0, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM, et al. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science. Jul 28, 1995;269(5223):496-512.
Gerth K, Bedorf N, Iirschik H, Hofle G, Reichenbach H. The soraphens: a family of novel antifungal compounds from Sorangium cellulosum (Myxobacteria). I. Soraphen Al alpha: fermentation, isolation, biological properties. J Antibiot (Tokyo). Jan. 1994;47(1):23-31.
Gil JA. Campelo-Diez AB. Candicidin biosynthesis in Streptomyces griseus. Appl Microbiol Biotechnol. 2003 Feb;60(6):633-42. Epub Dec. 18, 2002. Review.
Goryshin IY, Reznikoff WS. Tn5 in vitro transposition. J. Biol. Chem. 1998 273: 7367-74.
Haydock SF, Aparicio JP, Molnar I, Schwecke T, Khaw LE, Konig A, Marsden AF, Galloway IS, Staunton J., Leadlay PF. Divergent sequence motifs correlated with the substrate specificity of (methyl)malonylCoA:acyl carrier protein transacylase domains in modular polyketide synthases. FEBS Lett. Oct 30. 1995;374(2):246-8.
Hsieh YJ, Kolattukudy PE. Inhibition of erythromycin synthesis by disruption of malonyl-coenzyme a decarboxylase gene eryM in Saccharopolyspora erythraea. J Bacteriol. Feb. 1994;176(3):714-24.
Hunaiti AA, Kolattukudy PE. Source of methylmalonyl-coenzyme a for erythromycin synthesis: methylmalony-coenzyme a mutase from Streptomyces elythreus. Antimicrob Agents Chemother. Feb. 1984;25 (2):173-8.
Hu Z, Bao K., Zhou X, Zhou Q, Hopwood Da, Kieser T. Deng Z. Repeated polyketide synthase modules involved in the biosynthesis of a heptaene macrolide by Streptomyces sp. Fr-008. Mol Microbiol. 1994 Oct;14 (1):163-72.
Ikeda H, Nonomiya T, Usami M., Ohta T., Omura S. Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. Proc Natl Acad Sci U S a. 1999 Aug 17;96 (17):9509-14.
Kellermeyer Rw, Allen Shg, Stjernholm R, and Wood HG. Methylmalonyl isomerase. IV. Purification and properties of the enzyme from Propionibacteria. J. Biol. Chem. 1964 239:2562-2569.
Liu H, Reynolds Ka. Role of crotonyl coenzyme a reductase in determining the ratio of polyketides monensin a and monensin B produced by Streptomyces cinnamonensis. J Bacteriol. Nov. 1999;181(21):6806-13.
Marsh EN, McKie N, Davis NK, Leadlay PF. Cloning and structural characterization of the genes coding for adenosylcobalamin-45 dependent methylmalonyl-CoA mutase from Propionibacterium shermanii. Biochem J. Jun 1, 1989;260(2):345-52.
Miller ES. Cloning vectors, mutagenesis, and gene disruption (ermR) for the erythromycin-producing bacterium Aeromicrobium erythreum. Appl Environ Microbiol. Sep. 1991;57(9):2758-61.
Mochizuki S. Hiratsu K, Suwa M. Ishii T, Sugino F, Yamada K. Kinashi H. The large linear plasmid pSLA2- L of Streptomyces rochei has an unusually condensed gene organization for secondary metabolism. Mol Microbiol. Jun. 2003;48(6):1501-10.
Molnar I, Aparicio JF, Haydock SF, Khaw LE, Schweckee T, Konig A, Staunton J. Leadlay PF. Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. Gene. Feb 22, 1996;169(1):1-7.
OH SS, Chater KF. Denaturation of circular or linear DNA facilitates targeted integrative transformation of Streptomyces coelicolor A3(2): possible relevance to other 20 organisms. J Bacteriol. Jan. 1997;179(1):122-7.
Omura S, Tsuzki K, Tanaka Y, Sakakibara H, Aizawa M, Lukacs G. Valine as a precursor of n-butyrate unit in the biosynthesis of macrolide aglycone. J Antibiot (Tokyo). May 1983;36(5):614-6.
Omura S, Taki A, Matsuda K, Tanaka Y. Ammonium ions suppress the amino acid metabolism involved in the biosynthesis of protylonolide in a mutant ofStreptomyces fradiae. J Antibiot (Tokyo). 1984 Nov;37(11):1362-9.
Omura S, Takeshima H. Nakagawa A. Miyazawa J, Piriou F, Lukacs G. Studies on the biosynthesis of 16- membered macrolide antibiotics using carbon-13 nuclear magnetic resonance spectroscopy. Biochemistry. Jun. 28, 1977;16(13):2860-6.
Paulus TJ, Tuan JS, Luebke VE, Maine GT, Dewitt JP, Katz L. Mutation and cloning of etyG, the structural gene for erythromycin 0-methyltransferase from Saccharopolyspora erythraea, and expression of eryG in Escherichia coli. J Bacteriol. May 1990;172(5):2541-6.
Reeves AR, Weber G, Cernota WH, Weber JM. Analysis of an 8.1-kb DNA fragment contiguous with the erythromycin gene cluster of Saccharopolyspora erythraea in the eryCl-flanking region. Antimicrob Agents Chemother. Dec. 2002;46(12):3892-9.
Rodicio MR, Chater KR. Small DNA-free liposomes stimulate transfection of streptomyces protoplasts. J Bacteriol. Sep. 1982:151(3):1078-85.
Roberts AN, Barnett L, Brenner S. Transformation of Arthrobacter and studies on the transcription of the Arthrobacter ermA gene in Streptomyces lividans and Escherichia coli. Biochem J. Apr 15, 1987;243(2):431-6.
Rodriguez L. Aguirrezabalaga 1. Allende N. Brana Af, Mendez C. Salas Ja. Engineering deoxysugar biosynthetic pathways from antibiotic-producing microorganisms. A tool to produce novel glycosylated bioactive compounds. Chem Biol. Jun. 2002;9(6):721-9.
Schwecke T, Aparicio JF, Molnar I, Konig A. Khaw LE, Haydock

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