Process of generating transplastomic plants or plant cells...

Details Process of generating transplastomic plants or plant cells... Process of generating transplastomic plants or plant cells...

2008-05-13

2008-05-13

Kubelik, Anne (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

C435S320100

Reexamination Certificate

active

07371923

ABSTRACT:
A process of generating transgenic plants or plant cells transformed on their plastome and being devoid of a selection marker, comprising the following steps: (a) transforming plastids of a plant or plant cell with a DNA comprising: (i) a nucleotide sequence conferring replication of said DNA in a plant cell, (ii) at least one sequence of interest, (iii) sequences flanking said at least one sequence of interest necessary for stable integration of said at least one sequence of interest into the plastid genome, and (iv) a selection marker outside of said sequences flanking said sequence(s) of interest; (b) allowing integration of said at least one sequence of interest into the plastome in the presence of selective pressure; (c) allowing loss of said selection marker sequence by releasing selective pressure; and (d) recovering cells and/or plants being genetically transformed on their plastomes and being devoid of said selection marker.

REFERENCES:
patent: 5139812 (1992-08-01), Lebacq
patent: 5474925 (1995-12-01), Maliyakal et al.
patent: 5576198 (1996-11-01), McBride et al.
patent: 5627059 (1997-05-01), Capecchi et al.
patent: 5670623 (1997-09-01), Shoseyov et al.
patent: 5723765 (1998-03-01), Oliver et al.
patent: 5877402 (1999-03-01), Maliga et al.
patent: 6100448 (2000-08-01), Thompson et al.
patent: 6147278 (2000-11-01), Rogers et al.
patent: 6174700 (2001-01-01), Haynes et al.
patent: 6300545 (2001-10-01), Baszczynski et al.
patent: 6331416 (2001-12-01), Shani et al.
patent: 6331661 (2001-12-01), Baszczynski et al.
patent: 6781033 (2004-08-01), Staub et al.
patent: 2003/0188337 (2003-10-01), Day et al.
patent: 2004/0055037 (2004-03-01), Gleba et al.
patent: 2004/0083499 (2004-04-01), Eibl et al.
patent: 2004/0088764 (2004-05-01), Gleba et al.
patent: 2004/0137631 (2004-07-01), Herz et al.
patent: 2004/0191788 (2004-09-01), Gleba et al.
patent: 2004/0221330 (2004-11-01), Klimyuck et al.
patent: 2004/0244073 (2004-12-01), Klimyuck et al.
patent: 2004/0255347 (2004-12-01), Klimyuck et al.
patent: 2005/0014150 (2005-01-01), Atabekov et al.
patent: 2005/0015829 (2005-01-01), Koop et al.
patent: 2005/0015830 (2005-01-01), Dorokhov et al.
patent: 2005/0059004 (2005-03-01), Atabekov et al.
patent: 2005/0066384 (2005-03-01), Klimyuck et al.
patent: 2005/0091706 (2005-04-01), Klimyuck et al.
patent: 0270 248 (1988-06-01), None
patent: 1 045 037 (2000-10-01), None
patent: WO 87/00551 (1987-01-01), None
patent: WO 94/16089 (1994-07-01), None
patent: WO 95/34668 (1995-12-01), None
patent: WO 96/17954 (1996-06-01), None
patent: WO 98/09505 (1998-03-01), None
patent: WO 98/44097 (1998-10-01), None
patent: WO 98/54342 (1998-12-01), None
patent: WO 99/25821 (1999-05-01), None
patent: WO 99/25855 (1999-05-01), None
patent: WO 99/36516 (1999-07-01), None
patent: WO 01/11020 (2000-02-01), None
patent: WO 00/17365 (2000-03-01), None
patent: WO 00/20611 (2000-04-01), None
patent: WO 00/32799 (2000-06-01), None
patent: WO 00/68391 (2000-11-01), None
patent: WO 00/68431 (2000-11-01), None
patent: WO 00/70019 (2000-11-01), None
patent: WO 00/77174 (2000-12-01), None
patent: WO 00/77175 (2000-12-01), None
patent: WO 00/78985 (2000-12-01), None
patent: WO 01/59138 (2001-08-01), None
patent: WO 01/81600 (2001-11-01), None
patent: WO 02/12522 (2002-02-01), None
patent: WO 02/29068 (2002-04-01), None
patent: WO 02/46438 (2002-06-01), None
patent: WO 02/46440 (2002-06-01), None
patent: WO 02/055651 (2002-07-01), None
patent: WO 02/057466 (2002-07-01), None
patent: WO 02/068664 (2002-09-01), None
patent: WO 02/077246 (2002-10-01), None
patent: WO 02/079481 (2002-10-01), None
patent: WO 02/088369 (2002-11-01), None
patent: WO 02/101060 (2002-12-01), None
patent: WO 03/001900 (2003-01-01), None
patent: WO 03/004658 (2003-01-01), None
patent: WO 03/020927 (2003-03-01), None
patent: WO 03/020928 (2003-03-01), None
patent: WO 03/020938 (2003-03-01), None
El-Sheekh, 2000, Folia Microbiol. 45:496-504.
Mühlbauer et al, 2002, Plant J. 32:175-184.
Albert et al. (1995) “Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome” The Plant Journal 7:649-659.
Allison et al., “Deletion of rpoB Reveals a Second Distinct Transcription System in Plastids of Higher Plants,” The EMBO Journal, 15:11 2802-2809 (1996).
Altschul et al. “Basic Local Alignment Search Tool,” J. Mol. Biol., 215:403-410 (1990).
Anandalakshmi et al. (1998) “A viral suppressor of gene silencing in plants” Proc. Natl. Acad. Sci. U.S.A. 95:13079-13084.
Anandalakshmi et al. (2000) “A calmodulin-related protein that suppresses posttranscriptional gene silencing in plants” Science 290:142-144.
Arnold et al. “Allelic Ladder, D18S51 Allele 8” EBI Database accession No. AAX01351 (Apr. 14, 1999) Abstract.
Bagwell, BC “Poly-dA 50mer Probe Target Sequence” EBI Database accession No. AAQ66922 (Jan. 24, 1995) Abstract.
Bergamini et al. “Picornavirus IRESes and the Poly(A) tail Jointly Promote Cap-Independent Translation in a Mammalian Cell-free System,” RNA, 6:1781-1790 (2000).
Bogorad, Lawrence, “Engineering Chloroplasts: an Alternative Site for Foreign Genes, Proteins, Reactions and Products,” TIBTECH, 18:257-263 (Jun. 2000).
Bouchez et al. (1993) “A binary vector based on Basta resistance for in planta transformation of Arabidopsis thaliana” C. R. Acad. Sci. Paris, Science de la vie 316:1188-1193.
Boynton et al., “Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles,” Science, 240:1534-1538 (1988).
Carpin et al. (2001) “Identification of a Ca2+-Pectate Binding Site on an Apoplastic Peroxidase” The Plant Cell 13:511-520.
Chappell et al. “A 9-nt Segment of Cellular mRNA Can Function as an Internal Ribosome Entry Site (IRES) and When Present In Linked Multiple Copies Greatly Enhances IRES Activity,” PNAS, 97(4):1536-1541 (Feb. 15, 2000).
Clelland et al. (1999) “Hiding Messages in DNA Microdots,” Nature 399:533-534.
Coutts et al. “Development of Geminivirus-based Gene Vectors for Dicotyledonous Plants” Australian Journal of Plant Physiology 17:365-375 (1990).
Dale et al. “Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase” Gene 91:79-85 (1990).
Dale et al. “Mutations in the CRE/LOX Recombination Site Enhance the Stability of Recombination Products: Applications for Gene Targeting in Plants” Journal of Cellular Biochemistry 50 (S16S):206 (1992).
Daniell, “New Tools for Chloroplast Genetic Engineering,” Nature Biotechnology, 17:855-856 (Sep. 1999).
De Santis-Maciossek et al., “Targeted Disruption of the Plastid RNA Polymerase Genes rpoA, B and C1: Molecular Biology, Biochemistry and Ultrastructure,” The Plant Journal, 18(5):477-489 (1999).
Domingo et al. (1999) “Identification of a novel peptide motif that mediates cross-linking of proteins to cell walls” The Plant Journal 20:563-570.
Dorokhov et al. “Polypurine (A)-Rich Sequences Promote Cross-Kingdom Conservation of Internal Ribosome Entry” PNAS 99(8):5301-5306 (Apr. 16, 2002).
Drescher et al., “The Two Largest Chloroplast Genome-Encoded Open Reading Frames of Higher Plants are Essential Genes,” The Plant Journal, 22(2):97-104 (2000).
El-Sheekh, M.M. (2000) “Stable Chloroplast Transformation inChlamydomonas reinhardtiiusing Microprojectile Bombardment” Folia Microbiol. 45(6) 496-504.
Gatz et al. (1991) “Regulation of a modified CaMV 35S promoter by the Tn10-encoded Tet repressor in transgenic tobacco” Mol. Gen. Genet. 227:229-237.
Hager at al., “Enslaved Bacteria as New Hope for Plant Biotechnologists,” Appl. Microbiol. Biotechnol., 54:302-310 (2000).
Heifetz, Peter B., “Genetic Engineering of the Chloroplast,” Biochimie, 82:655-666 (2000).
Hoff et al. (2001) “A recombinase-mediated transcriptional induction system in transg

Affiliated with

Also associated with

Rating

Process of generating transplastomic plants or plant cells... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process of generating transplastomic plants or plant cells..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process of generating transplastomic plants or plant cells... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2813672