Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-02-08
2005-02-08
Riley, Jezia (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C210S634000, C423S658500
Reexamination Certificate
active
06852495
ABSTRACT:
The present invention provides a process of extracting nucleic acid in a simple manner at a high efficiency with no use of any hazardous reagent from biological materials having required specific procedure for nucleic acid extraction, particularly from biological materials such as bacteria of the genusMycobacterium;and a process of simultaneously carrying out the extraction and purification of nucleic acid in a simple manner from such biological materials existing in a biological sample such as feces having involved much difficulty in the purification of the nucleic acid. In the present invention, vigorous agitation of biological materials with fine particles in solutions containing chelating reagents or agitation of biological materials with aqueous solutions containing chelating reagents and quaternary ammonium salts, together with organic solvents and fine particles is performed.
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patent: 20020068292 (2002-06-01), Kojima
patent: 0338591 (1989-10-01), None
patent: 2002-037798 (2002-02-01), None
Vary et al, “Use of Highly Specific DNA Probes and the Polymerase Chain Reaction to DetectMycobacterium paratuberculosisin Johne's Disease” Journal of Clinical Microbiology, May 1990, p. 933-937 vol. 28, No. 5 American Society for Microbiology.
J.W.B. Van Der Giessen et al “Amplification of 16S rRNA sequences to detectMycobacterium paratuberculosis” J. Med. Microbiology, vol. 36 (1992) pp. 255-263 The Pathological Society of Great Britain and Ireland.
Agriculture Forestry and Fisheries Technical Information
Rader & Fishman & Grauer, PLLC
Riley Jezia
Shimadzu Corporation
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