Process of effecting a microinjection into living cells

Details Process of effecting a microinjection into living cells Process of effecting a microinjection into living cells

1989-02-21

1992-05-19

Warden, Robert J.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4351721, 435287, 935 53, 935 85, 7386425, C12N 1589

Patent

active

051148544

ABSTRACT:
For a microinjection of liquids or suspensions of substances into living cells, a stage and a cannula, which is movably mounted and extends at an oblique angle relative to the stage, are arranged to be movable relative to each other, the cells being contained in a culture vessel on the stage, the tip of the cannula constitutes a pointer and is moved in a plane z.sub.1, which is parallel to the stage and is disposed above the cells on the stage, the tip is moved to a position exactly over the location which is desired for an injection and thereafter, the cannula is moved away from the selected cell in the direction of the projection of the cannula on the plane and over a distance which depends on the angle between the cannula and the plane, the cannula is thus moved to a setback position, in which the axis of the cannula extends through the desired location, the cannula is then axially moved to puncture the cell and is subsequently reversed.

REFERENCES:
patent: 4532205 (1985-07-01), Ansorge
patent: 4619899 (1986-10-01), Nikitin et al.
patent: 4762405 (1988-08-01), Inoue et al.
patent: 4907158 (1990-03-01), Kettler et al.
"Micromanipulateurs steretaxigues dans le domaine chirurgical", Diaz et al., 8132 Revue Generale de l'Electricite, (1984), No. 11, Paris, France, pp. 747-751.

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