Process for treating yeast with B-1, 3-glucanase to produce micr

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

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424489, 426 62, 426656, 4284022, 435174, 435177, 435182, 435243, 4352554, 435259, C12N 116, C12N 1104, C12N 106, A61K 914

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055210899

ABSTRACT:
Microorganism cells such as Saccharomyces or Candida yeast cells are treated with an alkaline aqueous solution with heating and stirring or with a cell wall dissolving enzyme such as glucanase or mannase to obtain cells that function as microcapsules by rapidly taking up a large amount of hydrophobic liquid. Treatment with an alkaline aqueous solution is preferably carried out at a pH of at least 8 for at least 1 hour at a temperature of 20.degree.-100.degree. C. A preferred embodiment of enzyme treatment is with .beta.-1,3-glucanase for about 30 minutes to 5 hours at pH 4-9 at a temperature of 30.degree.-60.degree. C. The treatments dissolve cell walls of the yeast such that the walls still have sufficient strength for enclosing hydrophobic liquids.

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Rodriguez, R. L. et al. In: Recombinant DNA Techniques: An Introduction; Publisher: The Benjamin/Cummings Publishing Co. Inc; pp. 96-97, 1983.

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