Process for transforming gramineae and the products thereof

Details Process for transforming gramineae and the products thereof Process for transforming gramineae and the products thereof

1989-11-13

1993-02-16

Fox, David T.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352522, 4353201, 935 67, C12N 1500, C12R 141

Patent

active

051870738

ABSTRACT:
A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir.sup.+ Agrobacterium tumefaciens. Also, this same method wherein the vir.sup.+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir.sup.+ Agrobacterium tumefaciens, a transformed Gramineae plant derived from a seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA and a Gramineae plant whose cells contain a segment of T-DNA. Finally, there are provided transformed Gramineae derived from seedlings infected with vir.sup.+ Agrobacterium tumefaciens and transformed Gramineae derived from seedlings infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.

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