Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1990-09-05
1993-01-05
Fox, David T.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4352522, 4352523, 4353201, 800205, 800DIG56, 935 30, 935 56, 935 67, C12N 1500, C12R 141, A01H 104
Patent
active
051770105
ABSTRACT:
A method of producing transformed corn comprising making a wound in a corn seedling in an area of the corn seedling containing rapidly dividing cells and inoculating the wound with vir.sup.+ Agrobacterium tumefaciens. Also, this same method wherein the vir.sup.+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed corn pollen grain, a corn pollen grain produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens, a corn pollen grain produced by a plant grown from a seedling infected with vir.sup..degree. A. tumefaciens containing a vector comprising genetrically-engineered T-DNA, a corn pollen grain whose cells contain a segment of T-DNA, and corn derived from each of these pollen grains. There are also provided a transformed corn plant, a transformed corn plant derived from a corn seedling infected with vir.sup.+ Agrobacterium tumefaciens, a transformed corn plant derived from a corn seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, and a corn plant whose cells contain a segment of T-DNA. Finally, there are provided transformed corn derived from a corn seeding infected with vir.sup.+ Agrobacterium tumefaciens, and transformed corn derived from a corn seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.
REFERENCES:
Hernalsteens et al. 1984, EMBO J 3(13):3039-3041.
Hooykaas-Van Slogteren et al. 1984, Nature 311:763-764.
Lorz et al. 1985, Mol. Gen. Genet. 199:178-182.
Potrykus et al. 1985, Mol. Gen. Genet. 199:183-188.
de la Pena et al. 1981, Chromosoma 83(2):241-248.
Korohoda et al. 1979, Z. Phanzenphysiol. 94:95-99.
Goldman Stephen L.
Graves Anne C. F.
Fox David T.
University of Toledo
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