Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1991-01-18
1994-11-15
Martinell, James
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
536 237, 536 241, C12N 1500, C12N 1571, C12N 1572, C12N 1573
Patent
active
053647793
ABSTRACT:
The invention relates to a process for transforming cells and to a recombinant DNA molecule for use therein. Said recombinant DNA molecule is a multifunctional linker comprising a DNA sequence which is homologous to a part of the receptor genome, an operator sequence and either a sequence which allows replication in a microorganism or a sequence which allows for integrative recombination in a vector. Preferably, said multifunctional linker also contains a gene whose expression can be detected in a microorganism, a gene whose expression can be detected in the receptor, a sequence which promotes recombination, and/or a hairpin structure at one or both ends. The transformation process uses donor DNA coupled to the multifunctional linker and allows for early success followed by further characterization of the donor DNA and its phenotypic impact.
REFERENCES:
patent: 4506013 (1985-03-01), Hershberger et al.
patent: 4650761 (1987-03-01), Hershberger et al.
Reeck et al.; Cell 50: 667 (1987).
Smithies et al.; Nature 317: 230 (1985).
Yoneda et al.; Biochem. Biophys. Res. Comm. 91: 1556 (1979).
Smith et al.; Cell 24: 429 (1981).
Dawkins, The Extended Phenotype, 1982, Oxford University Press, Oxford, pp. 85, 86, and 287.
Martinell James
Solvay (Societe Anonyme) and Clovis Matton, N.V.
White John P.
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