Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1984-07-25
1989-11-14
Huleatt, Jayme A.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435320, 435255, 43525233, 435 91, 935 28, 935 29, 935 69, 935 73, 935 56, C12N 1500, C12N 116, C12N 120, C12P 1934
Patent
active
048807419
ABSTRACT:
Process for transformation of Yarrowia lipolytica, vectors useful therefor comprising DNA of a microbial vector and chromosomal DNA of Y. lipolytica and transformants comprising said vectors in E. coli and Y. lipolytica, and integrative shuttle vectors for Escherichia-Yarrowia transgeneric cloning. Said vectors or subclones thereof enable creation of Y. lipolytica cloning vectors into which specific or random segments of DNA can be inserted and the resulting vectors used to transform a suitable host microbe, especially Y. lipolytica, to improve the fermentation characteristics thereof and hence their industrial utilization.
The methodology described permits the cloning of genes from a gene library of Y. lipolytica by complementation with an integrating vector.
REFERENCES:
patent: 4394443 (1983-07-01), Weissman et al.
patent: 4407953 (1983-10-01), DeZeeuw et al.
patent: 4628033 (1986-12-01), DeZeeuw et al.
Davidow, L. et al., Curr. Genetics, vol. 10, pp. 39-48, 1985.
Ogrydziak, D. et al., J. Cell. Biochem., Supp. 9C, Abstracts, UCLA Symposia on Molecular & Cellular Biology, Apr. 6-25, Abstract #1622, p. 193, 1985.
Ogrydziak, D. et al., J. Gen. Microbiol., vol. 28, pp. 1225-1234, 1982.
Hinnen, A. et al., Proc. Natl. Acad. Sci., vol. 75, No. 4, pp. 1929-1933, 1978.
Beggs, J., Nature, vol. 275, pp. 104-109, 1978.
Ogrydziak, D. et al., Mol. Gen. Genet., vol. 188, pp. 179-183, 1982.
Van der Walt et al., Antonie Van Leeuwenhock, vol. 46, pp. 517-521, 1980.
Botstein, D. et al., in The Molecular Biology of the Yeast Saccharomyces, pp. 607 and 615-623, 1982, (Cold Spring Harbor Laboratory).
Mantiatis, T. et al., in Molecular Cloning, (Cold Spring Harbor Laboratory), pp. 3-5 and 13-15, 1982.
Hinnen et al., in "Overproduction of Microbiol Products", (Eds. Krumphanzl et al.), pp. 381-393 (1982), Academic Press, New York.
Ratzkin et al., Proc. Natl. Acad. Sci., 74, 487-491 (1977).
S. Das et al., Curr. Gent., 6, 123-128 (1982).
Beach et al., Nature, 290, No. 5802, 140-142 (1981).
Orr-Weaver et al., Proc. Natl. Acad. Sci., 78, 6354-6358 (1981).
Hinnen et al., in "Gene Cloning in Organisms Other than E. coli", Edited by Hofschneider and Goebel, pp. 101-106, Springer-Verlag (1982).
Davidow Lance S.
DeZeeuw John R.
Benson Gregg C.
Huleatt Jayme A.
Lumb J. Trevor
Pfizer Inc.
Richardson Peter C.
LandOfFree
Process for transformation of Yarrowia lipolytica does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for transformation of Yarrowia lipolytica, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for transformation of Yarrowia lipolytica will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1852719