Process for transducing Escherichia coli K12 .chi.1776

Details Process for transducing Escherichia coli K12 .chi.1776 Process for transducing Escherichia coli K12 .chi.1776

1978-06-02

1982-03-30

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435849, C12N 1500

Patent

active

043224970

ABSTRACT:
A method of providing maximum frequencies of transduction with Escherichia coli strains, such as E. coli K12 .chi.1776, that are transduced poorly in the exponential growth phase, which comprises transducing the culture in the stationary phase of growth. E. coli K12 .chi.1776 strains modified by transduction using this method are useful as EK2 hosts for biological containment.

REFERENCES:
patent: 4190495 (1980-02-01), Curtiss
Curtiss et al., Miami Winter Symp. 1977, 13, Mol Cloning Recomb. DNA, pp. 99-114.
Derwent Abstract 25578A (Abstracting German Offenlegungsschrift 26 44 432.

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