Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1984-02-06
1987-04-14
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435803, 426 2, 426 72, 210663, C12P 1942, A23K 116, A23L 1302, B01D 1500
Patent
active
046578595
ABSTRACT:
A process is disclosed for the treatment of fermentation broths containing vitamin B.sub.12 and other corrinoids, and for the preparation of vitamin B.sub.12 concentrates or crystalline vitamin B.sub.12 wherein the fermentation broth is contacted with a nonionic, macroreticular, adsorption resin having a pore size of 10.sup.-8 to 10.sup.-7 meters, a grain diameter of at least 10.sup.-4 m, and a specific surface area of at least 200 m.sup.2 /g so that the macromolecular adsorption resin adsorbs fermentation broth additives and metabolites of fermentation, whereas the intact microorganism cells containing the vitamin B.sub.12 remain in solution. The solution of intact vitamin B.sub.12 -containing cells is then treated further to obtain vitamin B.sub.12 in still purer form.
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Jaksa Istvan
Kelemen Agnes
Stefko Bela
Udvardy Nagy nee Cserey Pechany Eva
Dubno Herbert
Ross Karl F.
Weimar Elizabeth C.
Wiseman Thomas G.
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