Process for the synthesis of sugar nucleotides using recombinant

Details Process for the synthesis of sugar nucleotides using recombinant Process for the synthesis of sugar nucleotides using recombinant

1995-06-06

1998-10-20

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 435105, 4352523, 536 237, C12Q 168, C12P 2100, C12P 1906, C12N 121

Patent

active

058244727

ABSTRACT:
A recombinant-DNA mediated method for the synthesis of sugar nucleotides is disclosed. This method utilizes portable DNA sequences capable of directing the microbial synthesis of various enzymes that catalyze the synthesis of sugar nucleotides, including UDP-glucose, UDP-glucuronic acid and GDP-mannose. The sugar moieties of these sugar nucleotides may subsequently be incorporated into industrially-useful polysaccharides such as xanthan gum. It has been found that vectors containing the portable DNA sequences described herein are capable both of causing sugar nucleotide production in microorganisms previously incapable of such synthesis and of causing increased sugar nucleotide production in organisms capable of synthesizing small quantities of these compounds. In particular, plasmids pAS7, pAS9 and pTS13 are disclosed. These plasmids are capable of directing sugar nucleotide synthesis in various hosts, including Xanthomonas sp. such as X. campestris and other organisms such as E. coli and various Pseudomonas sp.

REFERENCES:
patent: 4296203 (1981-10-01), Wernau
patent: 4357423 (1982-11-01), Cox et al.
patent: 4407951 (1983-10-01), Weisrock et al.
patent: 4713449 (1987-12-01), Vanderslice et al.
U.S. application No. 07/333,285, Vanderslice et al., filed Apr. 5, 1989.
U.S. application No. 07/029,090, Doherty et al., filed Mar. 23, 1987.
U.S. application No. 07/384,621, Doherty et al., filed Jul. 25, 1989.
U.S. application No. 07/333,868, Capage et al., filed Apr. 3, 1989.
Buchanan et al., Journal of Bacteriology, 115:1011-1020 (1973).
E.S. Creeqer et al., J. Biol. Chem., 254:811-815 (1979).
R.P. Silver et al., Nature, 289:696-698 (1981).
S.K. Kadam et al., J. Bacteriol., 161:277-284 (1985).
A. Darzins et al., J. Bacteriol., 159:9-18 (1984).
A. Darzins et al., J. Bacteriol., 161:249-257 (1985).
J.B. Goldberg et al., J. Bacteriol., 158:1115-1121 (1984).
S. Gottesman et al., J. Bacteriol., 162:1111-1119 (1985).
R.C. Goldman et al., J. Bacteriol., 163:256-261 (1985).
Darzins et al., J. Bacteriol., 164:516-524 (1985).
Okita et al., J. Biol. Chem., 256:6944-6952 (1981).
Joyce et al., J. Biol. Chem., 257:1958-1964 (1982).
Gay et al., J. Biol., 153:1424-1431 (1983).
Ditta et al., Plasmid, 13:149-153 (1985).
Fishel et al., Develop. Biol., 110(2):369-381 (1985).
Couto et al., J. Biol. Chem., 259:378-382 (1984).
Creeger et al., J. Biol. Chem., 254:804-810 (1979).
Adhya et al., J. Bacteriol., vol. 108(2):621-626 (1971).
Kuriki et al., J. Biochem., vol. 58(3);308-311 (1965).
Daniels et al., EMBO J, vol. 3(13):3323-3328 (1984).
V. Deretic et al., Journal of Bacteriology, vol. 169, No. 1, pp. 351-358 (1987).
Souw et al., Appl. Environ. Microbiol., vol. 37, pp. 1186-1192.
European Search Report dated Mar. 28, 1997.

Affiliated with

Also associated with

Rating

Process for the synthesis of sugar nucleotides using recombinant does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for the synthesis of sugar nucleotides using recombinant, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the synthesis of sugar nucleotides using recombinant will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-242858