Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-06-07
1998-06-02
Marschel, Ardin H.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 231, 536 253, C07H 1900, C07H 2100
Patent
active
057602026
ABSTRACT:
Improved processes for the synthesis of 2'-O-substituted pyrimidine nucleosides are provided. The processes feature alkylation of a 2,2'-anhydropyrimidine nucleoside or a 2S,2'-anhydropyrimidine nucleoside with a weak nucleophile in the presence of a Lewis acid.
REFERENCES:
patent: 3687808 (1972-08-01), Merigan et al.
patent: 5210264 (1993-05-01), Yau
patent: 5386023 (1995-01-01), Sanghvi et al.
patent: 5470967 (1995-11-01), Huie et al.
Solomons "Organic Synthesis, Fifth Edition" John Wiley & Sons, Inc., p. 433, 1992.
March "Advanced Organiv Chemistry, Third Edition", John Wiley & Sons, Inc., p. 227, 1985.
Fraser et al. "Synthesis and Conformational Properties of 2'-Deoxy-2'-methylthio-pyrimidine and -purine Nucleosides: Potential Antisense Applications" J. Heterocyclic Chem., vol. 30, pp. 1277-1287, 1993.
Uhlmann et al. "Antisense Oligonucleotides: A New Therapeutic Principle" Chemical Review, vol. 90, pp. 543-584, 1990.
Sterzyck et al. "Synthesis and Anti-HIV Activity of several 2'-Fluoro-Containing Pyrimidine Nucleosides" J. Med. Chem., vol. 33, pp. 2150-2157, 1990.
Alul, R. et al., "Oxalyl-CPG: a Labile Support for Synthesis of Sensitive Oligonucleotide Derivatives", Nucleic Acids Research 1991, 19(7), 1527-1532.
Beaucage, S. and Iyer, "Advances in the Synthesis of Oligonucleotides by the Phosphoramidite Approach", Tetrahedron 1992, 48(12), 2223-2311.
Bielinska, A. et al., "Regulation of Gene Expression with Double-Stranded Phosphorothioate Oligonucleotides", Science 1990, 250, 997-1000.
Blackburn, G. and Gait, "Nucleic Acids in Chemistry and Biology", Chapter 3, p. 98, IRL Press, New York, 1990.
Bradford, M., "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding", Analytical Biochemistry 1976, 72, 248-254.
Coussens, L. et al., "Multiple, Distinct Forms of Bovine and Human Protein Kinase C Suggest Diversity in Cellular Signaling Pathways", Science 1986, 233, 859-866.
Dean, N. et al., "Inhibition of Protein Kinase C-.alpha. Expression in Human A549 Cells by Antisense Oligonucleotides Inhibits Induction of Intercellular Adhesion Molcelule 1 (ICAM-1) mRNA by Phorbol Esters", The J. of Biol. Chem. 1994, 269 (23), 16416-16424.
Englisch, U. and Gauss, "Chemically Modified Oligonucleotides as Probes and Inhibitors", Angew. Chem. Int. Ed. Engl. 1991, 30, 613-629.
Gibson, K. and Benkovic, "Synthesis and Application of Derivatizable Oligonucleotides", Nucleic Acids Research 1987, 15(16), 6455-6467.
Graham, F.G. and Van der Eb, "A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA", Virology 1973, 52, 456-467.
Greene, T. and Wuts, "Protective Groups in Organic Synthesis", John Wiley & Sons, New York, 2nd edition, 1991, Chap. 2, 6, 7.
Haralambidis, J. et al., "Preparation of Base-Modified Nucleosides Suitable for Non-Radioactive Label Attachment and Their Incorporation Into Synthetic Oligodeoxyribonucleotides", Nucleic Acids Research 1987, 15(12), 4857-4875.
Kroschwitz, J., ed., "Concise Encyclopedia of Polymer Science and Engineering", pp. 858-859, John Wiley & Sons, New York, 1990.
Manoharan, M., "Designer Antisense Oligonucleotides: Conjugation Chemistry and Functionality Placement", pp. 310-312 in Antisense Research and Applications, Crooke and Lebleu, eds., CRC Press, Boca Raton, 1993.
Miesfeld, R. et al., "Genetic Complementation of a Glucocoricoid Receptor Deficiency by Expression of a Cloned Receptor cDNA", Cell 1986, 46, 389-399.
Vorbruggen, H. et al., "A Simple New Synthesis of 2-Thiopyrimidine Nucleosides", Angew. Chem. Internat. Edit. 1969, 12, 976-977.
Vorbruggen, H. and Strehlke, "Eine einfache Synthese von 2-Thiopyrimidin-nucleosiden", Chem. Ber. 1973, 106, 3039-3061.
Wright, P. et al., "Large Scale Synthesis of Oligonucleotides via Phosphoramidite Nucleosides and a High-loaded Polystyrene Support", Tetrahedron Letters 1993, 34(21), 3373-3376.
Wu, H. et al., "Inhibition of In Vitro Transcription by Specific Double-Stranded Oligodeoxyribonucleotides", Gene 1990, 89, 203-209.
Borer, P.N. et al., "Stability of Ribonucleic Acid Double-Stranded Helices", J. Mol. Biol. 1974, 86, 843-853.
Damha, M.J. et al., "An Improved Procedure for Derivatization of Controlled-pore Glass Beads for Solid-phass Oligonucleotide Synthesis", Nucleic Acids Research 1990, 18, 3813-3821.
Freier, S.M. et al., in "Gene Regulation: Biology of Antisense RNA and DNA", Fox, C.F., ed., Raven Press, New York, 1992, pp. 95-107.
Freier, S.M. et al., "Solvent Effects on the Dynamics of (dG-dC0.sub.3 ", Biopolymers 1983, 22, 1107-1131.
Guinosso, C.J. et al., "Synthesis and Biophysical and Biological Evaluation of 2'-Modified Antisense Oligonucleotides", Nucleosides and Nucleotides 1991, 10, 259-262.
Lesnik, E.A. et al., "Oligodeoxynucleotides Containing 2'-O-Modified Adenosine: Synthesis and Effects on Stability of DNA:RNA Duplexes", Biochemistry 1993, 32, 7832-7838.
Monia, B.P. et al., "Selective Inhibition of Mutant Ha-ras mRNA Expression by Antisense Oligonucleotides", J. Biol. Chem. 1992, 267, 19954-19962.
Monia, B.P. et al., "Evaluation of 2'-Modified Oligonucleotides Containing 2'-Deoxy Gaps as Antisense Inhibitors of Gene Expression", J. Biol. Chem. 1993, 268, 14514-14522.
Petersheim, M. and Turner, "Base-Stacking and Base-Pairing Contributions to Helix Stability: Thermodynamics of Double-Helix Formations with CCGG, CCGGp, CCGGAp, ACCGGp, CCGGUp, and ACCGGUp", Biochemistry 1983, 22, 256-263.
Puglisi, J. and Tinoco, "Absorbance Melting Curves of RNA", Methods in Enzymology 1989, 180, 304-325.
Ueda, T. and Tanaka, "Synthesis of 2-Thiouridine and 6-Methyl-3-(.beta.-D-ribofuranosyl)-2-Thiouracil", Chem. Pharm. Bull. (Tokyo) 1970, 18, 1491-1493.
Morvan, et al., "Comparative Evaluation of Seven Oligonucleotide Analogues as Potential Antisense Agents," Jour. Med. Chem., 1993, vol. 36, pp. 280-287.
Kawasaki, et al., "Uniformly Modified 2'-Deoxy-2'-Fluoro Phosphorothioate Oligonucleotides as Nuclease-Resistant Antisense Compounds w/High Affinity & Specificity for RNA Targets", Jour. Med. Chem., 1993, vol. 36, pp. 831-841.
Wagner et al., "Antisense Gene Inhibition by Oligonucleotides Containing C-5 Propyne Pyrimidines," Science, 4 Jun. 1993, vol. 260, pp. 1510-1513.
Lamond, et al., Antisense Oligonucleotides Made of 2'-O-alkyl RNA: Their Properties & Applications in RNA Biochemistry. FEBS Letters, Jun. 1993, vol. 325, No., 1, 2, pp. 123-127.
Cook Phillip Dan
Ross Bruce S.
Sprankle Kelly G.
Springer Robert H.
ISIS Pharmaceuticals Inc.
Marschel Ardin H.
Riley Jezia
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