Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of...
Reexamination Certificate
2008-05-27
2008-05-27
Lilling, Herbert J. (Department: 1657)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
C435S135000, C528S491000, C528S493000
Reexamination Certificate
active
07378266
ABSTRACT:
Improved processes for extracting polyhydroxyalkanoate from a biomass containing the polyhydroxyalkanoate including the steps of: combining the biomass containing the polyhydroxyalkanoate with a solvent selected from lower chain ketones, and mixtures thereof to form a biomass liquor wherein the biomass liquor comprises less than about 25% water; mixing the biomass liquor for from about 10 to about 300 minutes at a temperature in the range of from about 70° C. to about 120° C.; separating the polyhydroxyalkanoate from the biomass liquor to form a PHA-enriched liquor, wherein the separating occurs at a temperature of at least about 40° C.; mixing the PHA-enriched liquor with water to form precipitated polyhydroxyalkanoate and an impure solvent liquor, wherein the water is mixed with the PHA-enriched liquor in the ratio of from at least about 0.4-1 parts of water to one part solvent; and recovering the precipitated polyhydroxyalkanoate from the impure solvent liquor. Optional treatment with oxidizing agents or mild surfactant or mild bleach is used to enhance color and odor.
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Cearley Angella Christine
Gibson Michael Steven
Narasimhan Karunakaran
Welling Stanley James
Lilling Herbert J.
Luedeka Neely & Graham P.C.
Meredian, Inc.
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