Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2005-06-14
2005-06-14
Prouty, Rebecca E. (Department: 1652)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S363000, C530S364000, C530S412000, C530S415000, C530S413000
Reexamination Certificate
active
06906183
ABSTRACT:
A process for the partial or complete separation of glycosylated and nonglycosylated proteins is described, in which: a) a triazine dye immobilized on a matrix is incubated with a mixture of glycosylated and nonglycosylated proteins, b) the matrix is then washed to remove the unbound proteins, and c) the proteins are eluted by means of a stepwise or continuous increase in the ionic strength or in the pH, nonglycosylated proteins and proteins having an increasing degree of glycosylation being collected separately from one another in the eluate fractions obtained. By means of the use of this process it is possible, for example, to prepare a human albumin which is free of glycoside bonds and is expressed in yeast cells.
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Feussner Annette
Römisch Jürgen
Stauss Harald
Weisse Jörg
Delta Biotechnology Ltd.
Finnegan Henderson Farabow Garrett & Dunner L.L.P.
Prouty Rebecca E.
Ramirez Delia M.
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