Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1983-05-17
1990-07-31
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435212, 530380, 530393, 530412, 530416, C12N 974, C12N 948
Patent
active
049450549
ABSTRACT:
The present invention relates to a process for separating and purifying proteases and antiproteases. This process is characterized in that there are placed in contact an insoluble cross-linked polymer including in its chain the group --SO.sub.3 R.sub.1 -- and/or the group --SO.sub.2 R.sub.2 -- in which R.sub.1 denotes a hydrogen atom or metal and R.sub.2 denotes the remainder of an amino acid linked to the --SO.sub.2 -- bridge through its amine --NH--, function, with the solution containing proteases and antiproteases or their complex; separating the polymer which has absorbed the desired protein, washing it carefully with the buffer, desorbing the absorbed protein by a solution of a polycationic compound in the case of T or by an albumin solution in the case of AT or of the complex T-AT, and isolating the protein, if desired, by known means, such as, especially, freeze drying. The invention is useful for studying the mechanism of blood coagulation.
REFERENCES:
patent: 3531463 (1970-09-01), Gustafson et al.
patent: 3902964 (1975-09-01), Greenspan
patent: 3928587 (1975-12-01), Sawyer
Fougnot, C. et al., Thrombosis Research, vol. 28, pp. 37-46, Oct. 1982.
Vesa, V. et al. (Uses, Nauchno-Issled, Inst. Prikl, Enzimol., Vilnius, USSR). Polifermentn. Sist., Tezisy. Soobshch. Uses.
Semin, "Perspekt. Primem. Polifermentn. Sist. Nar. Khoz." 1980, 1, 52-80, (Edited Klesov. A. A.)-English Translation.
Walsh et al., Methods in Enzymology, vol. XIX, (Eds Perlmann & Lorand) pp. 31-33, 1970.
Norde et al., J. Colloid and Interface Sci., vol. 66, No. 2, pp. 257-284, 1978.
Vesa, V. et al., Chem Abst. vol. 95: 92840m, 1981.
Norde et al., Chem Abst. vol. 89: 210631v, 1978.
Norde et al., Chem Abst. vol 89:210632w, 1978.
Norde et al., Chem., Abst., vol. 89: 210633x, 1978.
Norde et al., Chem., Abst., vol. 89: 210634y, 1978.
Fougnot Christine
Jozefowicz Marcel
Rosenberg Robert D.
Commissariat a l''Energie Atomique
Millstein Larry
Teskin Robin L.
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