Process for the selective separation of endoproteases

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435212, 435224, 435814, 252430, C12N 950, C12N 948, C12N 960

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active

043931391

ABSTRACT:
The present invention provides a process for the selective separation of endoproteases from aqueous solutions, wherein an aqueous solution containing proteases is treated with a complex, present in the solid phase, of alpha.sub.2 -macroglobulin with a divalent metal selected from zinc, cobalt, nickel and copper and the solid phase then separated off.
The present invention also provides an agent for carrying out this process, wherein it comprises a solid carrier material which is loaded with a complex of alpha.sub.2 -macroglobulin and of a divalent metal selected from zinc, cobalt, nickel and copper.

REFERENCES:
Trends in Biochemical Sciences (TIBS), vol. 5, pp. 43-47, 1980.
Analytical Biochemistry, vol. 99, pp. 415-420, (1979).
Biochemical Journal, vol. 133, pp. 709-724, (1973).
Methods in Enzymology, vol. XLV, pp. 639-652, (1976).

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