Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1981-08-28
1983-07-12
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435212, 435224, 435814, 252430, C12N 950, C12N 948, C12N 960
Patent
active
043931391
ABSTRACT:
The present invention provides a process for the selective separation of endoproteases from aqueous solutions, wherein an aqueous solution containing proteases is treated with a complex, present in the solid phase, of alpha.sub.2 -macroglobulin with a divalent metal selected from zinc, cobalt, nickel and copper and the solid phase then separated off.
The present invention also provides an agent for carrying out this process, wherein it comprises a solid carrier material which is loaded with a complex of alpha.sub.2 -macroglobulin and of a divalent metal selected from zinc, cobalt, nickel and copper.
REFERENCES:
Trends in Biochemical Sciences (TIBS), vol. 5, pp. 43-47, 1980.
Analytical Biochemistry, vol. 99, pp. 415-420, (1979).
Biochemical Journal, vol. 133, pp. 709-724, (1973).
Methods in Enzymology, vol. XLV, pp. 639-652, (1976).
Schrenk Jurgen
Wunderwald Peter
Boehringer Mannheim GmbH
Shapiro Lionel M.
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