Process for the scaleable purification of plasmid DNA

Details Process for the scaleable purification of plasmid DNA Process for the scaleable purification of plasmid DNA

2007-10-23

2007-10-23

Ketter, James (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S320100

Reexamination Certificate

active

10922324

ABSTRACT:
The present invention relates to a nonchromatographic-based process for the isolation of clinical grade plasmid DNA from bacterial cells. The exemplified methods described herein outline a scaleable, economically favorable protocol for the purification of clinical grade plasmid DNA fromE. coliwhich includes CTAB-based precipitation of DNA in combination with adsorption of impurities to calcium silicate.

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