Process for the removal of sucrose from a sugar mixture

Textiles: fiber preparation

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195 11, C12D 1302, C12D 1304

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active

041027434

ABSTRACT:
A culture medium composed of necessary sources and tap water is sterilized, inoculated under strains of the genus Streptococcus and then cultivated under aeration and agitation. Subsequent to the cultivation, the resultant was subjected to water-washing and sieving to obtain crude glucan. The crude glucan is then dissolved in NaOH solution and centrifuged. The recovered filtrate is neutralized with HCl and then the insolubilized glucan was collected by centrifugation. After repetition of the procedures, the collected glucan is washed with water thoroughly until no chloride ion is detected in the waste water and then dried to form white purified glucan powder.

REFERENCES:
ebisu et al., "Studies on the Structures of Polysaccharides (Glucans and Fructans) Produced by Cariogenic Streptococci and on an Enzyme Hydrolyzing the Insoluble Glucan-I. Structural Studies of Insoluble Glucan, Soluble Glucan and Fructans." Chemical Abstracts, vol. 85, No. 21, pp. 235, 236 (1976).
Ebisu et al., "The Structure of Water - Insoluble Glucans of Cariogenic Streptococcus Mutans, Formed in the Absence and Presence of Dextranase", Carbohydrate Research, vol. 38 (1974), pp. 374-381.
Baird et al., "Investigation of the Polysaccharides Produced by Extra cellular Glycosyltransferases from Streptococcus Mutans," Chemical Abstracts, Vol. 77, No. 11, p. 242 (1972).
Jeanes, et al., "Characterization and Classification of Dextrans From Ninety-Six strains of Bacteria," J. Amer. Chem. Soc., vol. 76, No. 20 (1954), pp. 5041-5052.

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