Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2011-01-18
2011-01-18
Crane, Lawrence E (Department: 1623)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
Reexamination Certificate
active
07872121
ABSTRACT:
Nitrogen-protecting groups are removed from the exocyclic nucleobase portion of a 2′-O protected nucleotide or 2′-halo nucleotide by contacting the nucleotide with an inorganic base. Typical is the removal of t-butylphenoxyacetyl protecting groups from the nucleobase portion of a 2′-O protected nucleotide on which the 2′-O protecting group is t-butyldimethylsilyl, removal or deprotection being accomplished by contact with a potassium carbonate solution.
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patent: 6664388 (2003-12-01), Nelson
(R) Schnetz-Boutaud et al., “Synthesis of Oligonucleotides Containing the Alkali-Labile Pyrimidopurinone Adduct, M1G,” Chemical Research in Toxicology, 13(2), 90-95 (2000).
(S) Fujimoto et al., “Synthesis and Cleavage of Oligonucleotides Containing a 5-Hydroxyuracil Residue in a Defined State,” Chemical Research in Toxicology, 10(11), 1254-1258 (1997).
N. D. Sinha et al, Labile Exocyclic Amine Protection (etc.), Biochimie, 1993, pp. 13-23, vol. 75, Elsevier, Paris.
Kuchimanchi Satya
Sinha Nanda Dulal
Avecia Biotechnology Inc.
Crane Lawrence E
Tinkler Timothy E.
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