Process for the reduction of star activities

Details Process for the reduction of star activities Process for the reduction of star activities

1991-03-14

1993-04-20

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435199, C12Q 168, C12N 924

Patent

active

052042383

ABSTRACT:
The present invention provides a process for the reduction of non-specific star activities in the case of the specific cleavage of desoxyribonucleic acids by incubation with a restriction endonuclease in an appropriate buffer, wherein to the incubation batch is added an antibiotic which binds to the DNA on or near the star sequences of the enzyme but not within the specific recognition sequence of the restriction endonuclease.

REFERENCES:
Pingoud, A. (1985) European Journal of Biochemistry vol. 147, pp. 105-109.
White, R. J. et al. (1989) Biochemistry, vol. 28, pp. 6259-6269.
Kopka, M. L. et al. (1985) P.N.A.S., vol. 82, pp. 1376-1380.
Low, C. M. L. et al. (1984) Nucleic Acids Research, vol. 12, pp. 4865-4879.
Berman, H. M. et al (1979) Biochimica and Biophysica Acta vol. 561, pp. 124-131.

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