Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1993-08-05
1994-08-23
Schwartz, Richard A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530300, 530305, 5303871, 530412, 530422, 435 711, 435 712, 435 691, 435226, 4352523, 43525233, C07K 312, C07K 1506
Patent
active
053409264
ABSTRACT:
In a process for the production of a soluble native protein, such as immunoglobulin or methionine-prochymosin, in which an insoluble form of the protein is produced by a host organism transformed with a vector including a gene coding for the protein, the insoluble form of the protein is reversibly denatured in an alkaline aqueous solution at a pH selected to promote dissociation of a group or groups of the protein involved in maintaining the conformation of the protein, and the protein is subsequently allowed to renature by reducing the pH of the solution below a pH effective to denature the protein to produce the soluble native form of the protein. The pH of the alkaline aqueous is suitably in the range 9.0 to 11.5.
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Angal Sarojani
Lowe Peter A.
Marston Fiona A. O.
Schoemaker Joyce A.
Celltech Limited
LeGuyader J.
Schwartz Richard A.
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