Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1991-03-29
1994-07-26
Schwartz, Richard A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530300, 530305, 530412, 530422, 435 711, 435 712, 435226, 4352523, 43525233, C07K 312, C07K 1506
Patent
active
053328051
ABSTRACT:
The production of recombinant chymosin is disclosed in which an insoluble form of chymosin precursor is produced by a bacterial host cell transformed by a vector including a coding sequence for said precursor. Solubilization of said insoluble form of chymosin precursor is accomplished using urea at a concentration of at least 7M or guanidine hydrochloride at a concentration of at least 6M prior to cleaving said precursor to form chymosin. Said solubilization preferably additionally involves the denaturation of said precursor in an alkaline aqueous solution, e.g., at a pH between a 9 and 11.5
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Carey Norman H.
Doel Michael T.
Harris Timothy J. R.
Lowe Peter A.
Celltech Limited
Le Guyader J.
Schwartz Richard A.
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