Process for the recovery of lipophilic proteins

Details Process for the recovery of lipophilic proteins Process for the recovery of lipophilic proteins

1995-06-07

1997-12-30

Guzo, David

Drug, bio-affecting and body treating compositions

Lymphokine

Interferon

530351, A61K 3821

Patent

active

057026998

ABSTRACT:
An improved process for recovering and purifying lipophilic recombinant proteins such as human .beta.-interferon from their hosts involves concentrating the bacteria; disrupting the cell wall and solubilizing the protein into an aqueous medium with an appropriate solubilizing agent; extracting the protein from the aqueous medium with 2-butanol, 2-methyl-2-butanol or mixtures thereof; precipitating and isolating the protein from the alcohol phase; purifying the protein by chromatography and diafiltering the protein against distilled water or aqueous solutions of ethanol or glycerol at a pH of about 12. Therapeutic formulations of such purified proteins with stabilizers contain SDS levels reduced to less than 10 p.p.m.

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