Process for the recovery of human .beta.-interferon-like polypep

Details Process for the recovery of human .beta.-interferon-like polypep Process for the recovery of human .beta.-interferon-like polypep

1983-05-18

1984-07-31

Kight, III, John

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

435 68, 435811, 424 85, C07G 700, A61K 4502

Patent

active

044629400

ABSTRACT:
An improved process for recovering and purifying .beta.-HIFN from transformed bacterial comprising concentrating the bacteria; disrupting the cell wall and solubilizing the .beta.-HIFN into an aqueous medium with an appropriate solubilizing agent; extracting the .beta.-HIFN from the aqueous medium with 2-butanol, 2-methyl-2-butanol or mixtures thereof; precipitating and isolating the .beta.-HIFN from the alcohol phase; purifying the .beta.-HIFN by chromatography and diafiltering the .beta.-HIFN against distilled water or aqueous solutions of ethanol or glycerol at a pH of about 12; and therapeutic formulations and compositions of .beta.-HIFN within SDS levels therein reduced to less than 10 p.p.m.

REFERENCES:
patent: 3800035 (1974-03-01), Goore
patent: 3855197 (1974-12-01), Hirsch et al.
patent: 4278661 (1981-07-01), Knight
patent: 4289689 (1981-09-01), Friesen et al.
patent: 4289850 (1981-09-01), Robinson
patent: 4315852 (1982-02-01), Leibowitz et al.
patent: 4343735 (1982-08-01), Menge et al.
patent: 4343736 (1982-08-01), Uemura et al.
Derynek et al., Expression of Human Fibroblast Interferon Gene in E. coli, Nature, 287, (1980), 193.
Scandella et al., A Membrane-Bound Phospholipase A1 Purified from E. coli, Biochemistry, 10(24), 1971, 4447.
U.S. Ser. No. 353,360 filed Mar. 1, 1982, entitled "Process for Recovering Human IFN-.beta. from a Transformed Microorganism".

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