Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1981-11-16
1983-01-11
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C12N 964
Patent
active
043682707
ABSTRACT:
A process for improving the recovery of enzyme coagulants from animal organs comprising the steps of adding a polar solvent having a surface tension in the range of about 18 to 72 dyne.cm.sup.-1 to the finely divided organs and thereafter adding salt solution to the organ/polar solvent mixture to extract the enzyme coagulants. Subsequently, the extract is activated by lowering the pH to a value in the range of 3.2 to 4.8 and gel-like substances such as mucin, are removed. The process results in an improved enzyme yield and minimizes the loss of enzyme activity to improve the stability.
REFERENCES:
patent: 2567378 (1951-09-01), Kennedy et al.
patent: 2701228 (1955-02-01), McKerns
patent: 3660237 (1972-05-01), Schultz
Shovers John
Weber Meyer M.
Midwest Biochemical Corp.
Shapiro Lionel M.
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