Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2001-05-29
2004-11-23
Gitomer, Ralph (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
C435S287300
Reexamination Certificate
active
06821722
ABSTRACT:
CROSS-REFERENCE TO RELATED APPLICATIONS
(NOT APPLICABLE)
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
(NOT APPLICABLE)
INCORPORATION-BY-REFERENCE OF MATERIAL SUBMITTED ON A COMPACT DISC
(NOT APPLICABLE)
BACKGROUND OF THE INVENTION
(1) Field of the Invention
The present invention relates to a device for the rapid measurement of an enzymatic activity in a solid feed, comprising (i) a container designed to contain the test sample, (ii) a reagent specific for the enzyme whose activity it is desired to measure, and (iii) a buffer for dissolving the enzyme.
The feed is preferably a solid feed which is not treated prior to the measurement.
(2) Description of Related Art
Feeds intended for husbandry animals are usually supplemented with enzymes whose role is mainly to improve the digestibility of the feed ration. These enzymes are usually sprayed in liquid from onto the feeds, in particular as described in patent EP 0,789,291. The enzymes can also be added in powder form to the feed.
Two problems thus arise, the first being to check the uniformity of distribution of the enzymes added to the feed, the second being to quickly and easily evaluate the activity of the enzyme(s) added to the feeds. These problems are raised in particular by feed manufactures and breeders wishing to check the quality of the feeds they want to give to their animals. Until now, the enzymatic activity could be measured in the laboratory, thus entailing constraints in terms of logistics and delays, these constraints being a real hindrance when an immediate result is needed.
BRIEF SUMMARY OF THE INVENTION
The present invention satisfies this problem by providing a device for measuring the enzymatic activity of any enzyme-enriched feed intended for animal feed. This device, whose measurement is based on a colorimetric reaction, allows both a qualitative measurement of the enzymatic activity of the test sample and a semi-quantitative measurement of this sample.
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Bio-Rad Life Science Research Products Catalogue, Price List S, 1993, p. 62.*
Megazyme (www.megazyme.com), endo-1,4-b-xylanase assay procedures, p. 6.*
Sabatier A. Method of Analysis for Feed Enzymes. J of Applied Poultry Research, 1996 5(4)408-413.*
Walsh G. Technical Note: Detection and Quantification of Supplemental Fungal Beta Glucanase Activity in Animal Feed. J Animal Science 1995 73(4)1074-1076.*
Burianova T. Assay of Very Low Cellulolytic Activity in Fodder Supplemented with Enzyme Preparation. Animal Feed Science and Technology 1991, 33(1-2)41-48.*
Heil, K. Assay of Enzyme Activity in Feeds. Zootecnica International 1997 20(3)40-43.
Moores Janet
Roberts Neil
Adisseo France S.A.S.
Connolly Bove & Lodge & Hutz LLP
Gitomer Ralph
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