Process for the rapid and simple isolation of nucleic acids

Details Process for the rapid and simple isolation of nucleic acids Process for the rapid and simple isolation of nucleic acids

1981-08-31

1989-05-16

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Lysis of micro-organism

435264, 435267, 435270, 435272, 435320, 435820, 426 60, 536 27, 530344, 530412, 530417, 530419, 530423, 530820, C12N 106, C12N 108, C07G 1700, C07K 312

Patent

active

048309699

ABSTRACT:
A process for the separation from other cellular materials of heat agglomeration resistant water soluble nitrogen containing organic compounds such as plasmids, RNA's, mitochondrial DNA's, viral DNA's, chloroplast DNA's, other episomal DNA's and certain proteins. The process comprises heating cellular materials in a solution of lysing agent to lyse the desired cells and to agglomerate water soluble nitrogen containing compounds such as certain chromosomal DNA's which are not resistant to agglomeration; centrifuging the resulting product to remove water soluble agglomerated materials; separating the supernatant liquid and precipitating the water soluble agglomeration resistant organic compounds with a water soluble precipitant. The process also includes separating the agglomeration resistant water soluble nitrogen containing compounds from each other by means of exclusion chromotography.

REFERENCES:
patent: Re25785 (1965-06-01), Beers
patent: 3585179 (1971-06-01), Samejima et al.
patent: 3681195 (1972-08-01), Suekane et al.
patent: 3930956 (1976-01-01), Juni
patent: 3934039 (1976-01-01), Cardini et al.
patent: 3959246 (1976-05-01), Bickoff et al.
patent: 3991215 (1976-11-01), Robbins
patent: 4038143 (1977-07-01), Juni
patent: 4260644 (1981-04-01), Eriksson et al.
patent: 4338400 (1982-07-01), Manis et al.
Clewell et al, PNAS USA, 62: 1159-1166 (1969).
Hirt, J. Molec. Biol., 26: 365-369 (1967).
Zasloff et al, Nucleic Acids Research, 5: 1139-1152 (1978).
Colman et al, Eur. J. Biochem., 95: 303-310 (1978).
Birnboim et al, Nucleic Acids Research, 7: 1513-1523 (1979).
Currier et al, Anal. Biochem., 76, 431-441 (1976).
Khoury et al, Methods in Enzymology, vol. LVIII, Academic Press, New York, 404, 406-410 (1979).
Longacre et al, Methods in Enzymology, 68, Academic Press, New York, 192-199 (1979).

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