Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-07-07
1992-03-10
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 724, 435967, 436 10, 436527, 436533, 436534, G01N 33546, G01N 33552
Patent
active
050949403
ABSTRACT:
A process for quantification of cell populations or subpopulations, by incubating a sample with labelled antibodies directed against characteristic surface antigens of the cell population to be quantified to form labelled antibody/antigen complexes. Standards with known, differing particle concentration and having comparable sedimentation behaviour to the cells to be determined and further, carrying molecules which are directed against the labelled antibody or a part hereof are also incubated with the labelled antibodies. The cells of the sample solution, as well as the particles of the standard solution, are separated off from the excess labelled antibodies and the amount of the labelling is measured not only on the cells but also on the particles. By comparison of the measurement value from the sample with the measurement values from the standard solutions, there is ascertained the number of cells to be determined in the sample. The standard and a reagent kit for carrying out the process are also the subject of this application.
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patent: 4745075 (1988-05-01), Hadfield et al
patent: 4828984 (1989-05-01), Schwartz
K. Shortman, Meth. Enzymol., 108, 110, 1984.
Albert Winfried
Endl Josef
Schetters Hartmut
Boehringer Mannheim GmbH
Saunders David
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