Process for the quantification of cell populations or subpopulat

Details Process for the quantification of cell populations or subpopulat Process for the quantification of cell populations or subpopulat

1986-11-05

1989-10-24

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 18, 435 21, 435 25, 435 28, 435 29, 435 34, 435810, 436 10, 436519, 436523, 436527, 436533, 436534, 436548, G01N 33545, G01N 33547, G01N 33552, G01N 33577

Patent

active

048761893

ABSTRACT:
A process for quantification of cell populations or subpopulations, by incubating a sample with labelled antibodies directed against characteristic surface antigens of the cell population to be quantified to form labelled antibody/antigen complexes. Standards with known, differing particle concentration and having comparable sedimentation behavior to the cells to be determined and further, carrying molecules which are directed against the labelled antibody or a part hereof are also incubated with the labelled antibodies. The cells of the sample solution, as well as the particles of the standard solution, are separated off from the excess labelled antibodies and the amount of the labelling is measured not only on the cells but also on the particles. By comparison of the measurement value from the sample with the measurement values from the standard solutions, there is ascertained the number of cells to be determined in the sample. The standard and a reagent kit for carrying out the process are also the subject of this application.

REFERENCES:
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patent: 4418152 (1983-11-01), Hosaka et al.
patent: 4486530 (1984-12-01), David et al.
patent: 4714682 (1987-12-01), Schwartz
Johnson et al., In Weir(ED.), Handbook of Experimental Immunology, 3rd Ed., Blackwell Scientific Publications, Oxford, 1978, pp. 15.1-15.17.
Nibbering et al., in Bullock et al., (EDS.), Techniques in Immunocytochemistry, vol. 3, Academic Press, London, 1982, pp. 97-113.
Knapp, in Wick et al., (EDS.), Immunofluorescence Technology, Elsevier Biomedical Press, Amsterdam, 1982, pp. 12-25.

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