Process for the quantative determination of free thyroxine

Details Process for the quantative determination of free thyroxine Process for the quantative determination of free thyroxine

1985-11-13

1989-06-27

Rosen, Sam

Chemistry: analytical and immunological testing

Thyroid hormone tests

436518, 436807, G01N 33543

Patent

active

048430188

ABSTRACT:
The present invention provides a process for the quantitative determination of free thyroxine (FT.sub.4) in plasma, serum or whole blood by immunological methods, wherein the sample is incubated for at most 10 minutes with a 10 to 2000 fold insufficiency of labelled anti-T.sub.4 antibodies, referred to the molar amount of total T.sub.4 in the sample, then immediately brought together with immobilized excess T.sub.4, again incubated, the phases separated and the label measured in one of the phases.

REFERENCES:
patent: 4017597 (1977-04-01), Reynolds
patent: 4067959 (1978-01-01), Bolz
patent: 4292296 (1981-09-01), Parsons, Jr.
patent: 4366143 (1982-12-01), Midgley et al.
patent: 4410633 (1983-10-01), Hertl et al.
patent: 4477576 (1984-10-01), Deutsch et al.
C. N. Hales et al, Meth. Enzymol., 70, 334-355, 1980.
Coat-A-Count Free Thyroxine, Diagnostic Products Corporation, 1983.

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